PMID- 9428359
OWN - NLM
STAT- MEDLINE
DCOM- 19980202
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 100
IP  - 2
DP  - 1998 Jan 15
TI  - Detection of trisomy 12 and centromeric alterations in CLL by interphase- and 
      metaphase-FISH.
PG  - 148-51
AB  - We have studied trisomy 12 in chronic lymphocytic leukemia (CLL) by fluorescence 
      in situ hybridization (FISH) with an alpha-satellite centromeric probe for 
      chromosome 12 on both dividing and non-dividing cells. Trisomy for chromosome 12 
      was demonstrated in four of these patients (15.3%) using FISH on interphase 
      cells. The percentage of trisomic cells ranged from 10% to 65% of nuclei. The 
      hybridization signals in the trisomic and disomic nuclei were of a broadly 
      similar size and nature. Interestingly, three of the remaining CLL patients, who 
      exhibited disomy for chromosome 12, showed a marked difference in size of the 
      hybridization signals in interphase nuclei. This was also demonstrated in 
      metaphase spreads. In addition, metaphase FISH studies revealed a supernumerary 
      marker chromosome in three out of 26 patients with CLL.
FAU - Acar, H
AU  - Acar H
AD  - Department of Medical Genetics, University of Selcuk, Konya, Turkey.
FAU - Connor, M J
AU  - Connor MJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - *Metaphase
MH  - *Trisomy
EDAT- 1998/01/15 00:00
MHDA- 1998/01/15 00:01
CRDT- 1998/01/15 00:00
PHST- 1998/01/15 00:00 [pubmed]
PHST- 1998/01/15 00:01 [medline]
PHST- 1998/01/15 00:00 [entrez]
AID - S0165-4608(97)00024-1 [pii]
AID - 10.1016/s0165-4608(97)00024-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Jan 15;100(2):148-51. doi: 
      10.1016/s0165-4608(97)00024-1.