PMID- 9428804
OWN - NLM
STAT- MEDLINE
DCOM- 19980130
LR  - 20071115
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 174
IP  - 2
DP  - 1998 Feb
TI  - Genetically recessive mutant of human monocytic leukemia U937 resistant to tumor 
      necrosis factor-alpha-induced apoptosis.
PG  - 179-85
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that induces apoptosis in 
      various cell systems by binding to the TNF receptor (TNFR). To study 
      TNF-alpha-induced apoptosis, we isolated and characterized a novel 
      TNF-alpha-resistant variant, U937/TNF clone UA, from human monocytic leukemia 
      U937 cells. The UA cells resist apoptosis induced by TNF-alpha and anti-Fas 
      antibody but not by anticancer drugs, such as VP-16 and Ara-C. Somatic cell 
      hybridization between U937 and UA showed that apoptosis resistance to TNF-alpha 
      in UA was genetically recessive. The hybridization analysis also showed that UA 
      and another recessive mutant clone, UC, belong to different complementation 
      groups in TNF-alpha-induced apoptosis signaling. In UA cells, TNF-alpha-induced 
      disruption of mitochondrial membrane potential and CPP32 activation were 
      abrogated. Expression of TNFR, Fas, and Bcl-2 family proteins was not changed in 
      UA cells. These results suggest that the apoptosis resistant UA cells could have 
      a functional defect in apoptosis signaling from the TNFR to mitochondria and 
      interleukin-1beta converting enzyme (ICE) family protease activation. UA cells 
      could be used to study signaling linkage between cell death-inducing receptor and 
      mitochondria.
FAU - Dong, J
AU  - Dong J
AD  - Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
FAU - Naito, M
AU  - Naito M
FAU - Mashima, T
AU  - Mashima T
FAU - Jang, W H
AU  - Jang WH
FAU - Tsuruo, T
AU  - Tsuruo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Apoptosis/drug effects/*genetics
MH  - Genes, Recessive
MH  - Humans
MH  - Leukemia, Myeloid/*genetics/pathology
MH  - *Mutation
MH  - Signal Transduction/drug effects
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1998/01/15 04:20
MHDA- 2000/06/20 09:00
CRDT- 1998/01/15 04:20
PHST- 1998/01/15 04:20 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/01/15 04:20 [entrez]
AID - 10.1002/(SICI)1097-4652(199802)174:2<179::AID-JCP5>3.0.CO;2-L [pii]
AID - 10.1002/(SICI)1097-4652(199802)174:2<179::AID-JCP5>3.0.CO;2-L [doi]
PST - ppublish
SO  - J Cell Physiol. 1998 Feb;174(2):179-85. doi: 
      10.1002/(SICI)1097-4652(199802)174:2<179::AID-JCP5>3.0.CO;2-L.