PMID- 9428806
OWN - NLM
STAT- MEDLINE
DCOM- 19980130
LR  - 20061115
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 174
IP  - 2
DP  - 1998 Feb
TI  - Differential effects of various growth factors and cytokines on the syntheses of 
      DNA, type I collagen, laminin, fibronectin, osteonectin/secreted protein, acidic 
      and rich in cysteine (SPARC), and alkaline phosphatase by human pulp cells in 
      culture.
PG  - 194-205
AB  - The purpose of this study is to differentiate roles of several growth factors and 
      cytokines in proliferation and differentiation of pulp cells during development 
      and repair. In human pulp cell cultures, laminin and type I collagen levels per 
      cell remained almost constant during the whole culture period (22 days). On the 
      other hand, secreted protein, acidic and rich in cysteine (SPARC/osteonectin) and 
      alkaline phosphatase (ALPase) levels markedly increased after the cultures 
      reached confluence. Laminin and type I collagen, as well as fibronectin, 
      stimulated the spreading of pulp cells within 1 h. Adding transforming growth 
      factor-beta (TGF-beta) decreased laminin and ALPase levels, whereas it increased 
      SPARC and fibronectin levels 3- to 10-fold. Western and Northern blots showed 
      that TGF-beta enhanced SPARC synthesis at the protein and mRNA levels. Basic 
      fibroblast growth factor (bFGF) decreased type I collagen, laminin, SPARC, and 
      ALPase levels without changing the fibronectin level. Platelet-derived growth 
      factor (PDGF) selectively decreased laminin, SPARC, and ALPase levels. Epidermal 
      growth factor (EGF) also decreased SPARC and ALPase levels. Tumor necrosis 
      factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) decreased type I 
      collagen and laminin levels, and abolished SPARC and ALPase syntheses. Of these 
      peptides, bFGF and PDGF showed the greatest stimulation of [3H]thymidine 
      incorporation into DNA. TGF-beta, EGF, and TNF-alpha had less effect on DNA 
      synthesis, whereas IL-1beta inhibited DNA synthesis. These findings demonstrated 
      that TGF-beta, bFGF, EGF, PDGF, TNF-alpha, and IL-1beta have characteristically 
      different patterns of actions on DNA, laminin, type I collagen, fibronectin, 
      ALPase, and SPARC syntheses by pulp cells.
FAU - Shiba, H
AU  - Shiba H
AD  - Department of Endodontology and Periodontology, Hiroshima University School of 
      Dentistry, Japan.
FAU - Fujita, T
AU  - Fujita T
FAU - Doi, N
AU  - Doi N
FAU - Nakamura, S
AU  - Nakamura S
FAU - Nakanishi, K
AU  - Nakanishi K
FAU - Takemoto, T
AU  - Takemoto T
FAU - Hino, T
AU  - Hino T
FAU - Noshiro, M
AU  - Noshiro M
FAU - Kawamoto, T
AU  - Kawamoto T
FAU - Kurihara, H
AU  - Kurihara H
FAU - Kato, Y
AU  - Kato Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cytokines)
RN  - 0 (Fibronectins)
RN  - 0 (Growth Substances)
RN  - 0 (Laminin)
RN  - 0 (Osteonectin)
RN  - 9007-34-5 (Collagen)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/*biosynthesis
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Collagen/*biosynthesis
MH  - Cytokines/*pharmacology
MH  - DNA/*biosynthesis
MH  - Dental Pulp/*metabolism
MH  - Fibronectins/*biosynthesis
MH  - Growth Substances/*pharmacology
MH  - Humans
MH  - Laminin/*biosynthesis
MH  - Osteonectin/*biosynthesis
EDAT- 1998/01/15 04:20
MHDA- 2000/06/20 09:00
CRDT- 1998/01/15 04:20
PHST- 1998/01/15 04:20 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/01/15 04:20 [entrez]
AID - 10.1002/(SICI)1097-4652(199802)174:2<194::AID-JCP7>3.0.CO;2-J [pii]
AID - 10.1002/(SICI)1097-4652(199802)174:2<194::AID-JCP7>3.0.CO;2-J [doi]
PST - ppublish
SO  - J Cell Physiol. 1998 Feb;174(2):194-205. doi: 
      10.1002/(SICI)1097-4652(199802)174:2<194::AID-JCP7>3.0.CO;2-J.