PMID- 9430373
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 135
IP  - 2
DP  - 1997 Dec
TI  - Apoptotic cell death in atherosclerotic plaques of hyperlipidemic knockout mice.
PG  - 235-9
AB  - In an attempt to understand the roles of apoptosis in the development of 
      atherosclerosis, we classified lesions developed in the aortas of apo E- and LDL 
      receptor-deficient mice, murine models of atherosclerosis, and determined 
      frequency, spatial distribution and cell types of apoptotic cells in each lesion. 
      Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and 
      nuclear staining with propidium iodide were used to demonstrate apoptotic cells. 
      Mean frequencies of TUNEL-positive cells were as follows: 0%, in Type I, 0.3% in 
      Type II, 0.05% in Type III, 0.06% in Type IV and 0.06% in Type V lesions. Most of 
      the TUNEL-positive cells were filled with fat and distributed in close proximity 
      to lipid pools. The TUNEL-positive cells in the intimal side of the lipid cores 
      were macrophages, while some of those in the adventitial side were smooth muscle 
      cells. In conclusion, apoptosis is involved in the active turn-over of foam cells 
      of both macrophage- and smooth muscle cell-lineage especially in the early 
      atherosclerotic lesions of the hyperlipidemic mice.
FAU - Harada, K
AU  - Harada K
AD  - The Third Department of Internal Medicine, Faculty of Medicine, University of 
      Tokyo, Japan.
FAU - Chen, Z
AU  - Chen Z
FAU - Ishibashi, S
AU  - Ishibashi S
FAU - Osuga, J
AU  - Osuga J
FAU - Yagyu, H
AU  - Yagyu H
FAU - Ohashi, K
AU  - Ohashi K
FAU - Yahagi, N
AU  - Yahagi N
FAU - Shionoiri, F
AU  - Shionoiri F
FAU - Sun, L
AU  - Sun L
FAU - Yazaki, Y
AU  - Yazaki Y
FAU - Yamada, N
AU  - Yamada N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Receptors, LDL)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Arteriosclerosis/genetics/metabolism/*pathology/physiopathology
MH  - Cell Death/genetics
MH  - DNA Nucleotidylexotransferase/analysis
MH  - Diet, Atherogenic
MH  - Female
MH  - Hyperlipidemias/genetics/metabolism/*pathology/physiopathology
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Receptors, LDL/deficiency/genetics
EDAT- 1998/01/16 00:00
MHDA- 1998/01/16 00:01
CRDT- 1998/01/16 00:00
PHST- 1998/01/16 00:00 [pubmed]
PHST- 1998/01/16 00:01 [medline]
PHST- 1998/01/16 00:00 [entrez]
AID - S0021-9150(97)00167-6 [pii]
AID - 10.1016/s0021-9150(97)00167-6 [doi]
PST - ppublish
SO  - Atherosclerosis. 1997 Dec;135(2):235-9. doi: 10.1016/s0021-9150(97)00167-6.