PMID- 9430491
OWN - NLM
STAT- MEDLINE
DCOM- 19980203
LR  - 20180213
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 115
IP  - 1
DP  - 1998 Jan
TI  - Intradermal injection of monocyte chemoattractant protein-1 induces emigration 
      and differentiation of blood monocytes in rat skin.
PG  - 15-23
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is a potent 
      chemoattractant for blood monocytes in vitro. Recent studies in MCP-1-transgenic 
      mice revealed that the local production of MCP-1 caused monocyte infiltration. 
      However, the kinetics of monocyte infiltration after the production of MCP-1 or 
      the amount of MCP-1 necessary for monocyte recruitment are not known. METHODS: We 
      purified recombinant rat MCP-1 expressed in COS-7 cells, and injected it into rat 
      skin. The infiltrating cells were examined by immunohistochemistry and 
      ultrastructural peroxidase cytochemistry. RESULTS: Rat recombinant MCP-1 had a 
      molecular mass of approximately 30 kD and exhibited the peak monocyte chemotactic 
      activity at 10(-9) M. One microgram of MCP-1 caused intra- and extravascular 
      accumulation of mononuclear cells 3 h after injection. The cells were ED1+, 
      indicating they were blood monocytes. The infiltration of mononuclear cells 
      peaked at 12-24 h, and most of them were TRPM-3+ and ED3+, characteristic to 
      exudate macrophages. None of the cells expressed ED2 or Ki-M2R antigens, markers 
      for resident macrophages, until 3 days after injection. There was no uptake of 
      [3H]thymidine by the infiltrating cells. Ultrastructural peroxidase cytochemistry 
      confirmed that the infiltrating cells were monocytes and exudate macrophages. The 
      number of OX8+ lymphocytes also peaked at 12 h, consisting of approximately 9% of 
      the total infiltrating cells. CONCLUSION: These results indicate that MCP-1 
      attracts blood monocytes as early as 3 h and the infiltrating monocytes 
      differentiate into exudate macrophages in loco. However, this effect was 
      transient and the infiltration of monocytes did not result in tissue damage.
FAU - Yamashiro, S
AU  - Yamashiro S
AD  - Second Department of Pathology, Kumamoto University School of Medicine, Japan.
FAU - Takeya, M
AU  - Takeya M
FAU - Kuratsu, J
AU  - Kuratsu J
FAU - Ushio, Y
AU  - Ushio Y
FAU - Takahashi, K
AU  - Takahashi K
FAU - Yoshimura, T
AU  - Yoshimura T
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Blood Vessels/immunology
MH  - COS Cells
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*immunology/isolation & purification
MH  - Chemotaxis, Leukocyte
MH  - Immunohistochemistry
MH  - Leukocytes/cytology/immunology
MH  - Lymphocytes/cytology/immunology
MH  - Macrophages/immunology/ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Monocytes/cytology/*immunology/ultrastructure
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins/immunology/isolation & purification/metabolism
MH  - Skin/immunology/pathology/ultrastructure
MH  - Specific Pathogen-Free Organisms
EDAT- 1998/01/16 05:44
MHDA- 2000/10/06 11:01
CRDT- 1998/01/16 05:44
PHST- 1998/01/16 05:44 [pubmed]
PHST- 2000/10/06 11:01 [medline]
PHST- 1998/01/16 05:44 [entrez]
AID - iaa15015 [pii]
AID - 10.1159/000023825 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 1998 Jan;115(1):15-23. doi: 10.1159/000023825.