PMID- 9431456
OWN - NLM
STAT- MEDLINE
DCOM- 19980223
LR  - 20190921
IS  - 0386-7196 (Print)
IS  - 0386-7196 (Linking)
VI  - 22
IP  - 5
DP  - 1997 Oct
TI  - Characteristic expression of high molecular mass heat shock protein HSP105 during 
      mouse embryo development.
PG  - 517-25
AB  - Expression of 105 k-Da heat shock protein (HSP105) during mouse embryo 
      development was investigated. Although HSP105 was detected scarcely in mouse 
      embryos at gestational day (GD) 8, its level of HSP105 increased markedly in the 
      embryos from GD 9 to 11, and then decreased gradually by GD 14. In contrast, 70 
      k-Da heat shock cognate protein (HSC70) was detected at constantly high levels in 
      embryo from GD 8 to 17 (before birth). The transient increase in level of HSP105 
      was observed in most embryonic tissues. Since the levels of HSP105 mRNA was 
      detected at constantly high levels in the embryos between GD 8 and 12, the 
      increased expression of HSP105 at GD 9 to 11 seemed to be regulated at 
      posttranscriptional level. Immunohistochemically, HSP105 as well as HSC70 were 
      localized in the cytoplasm and the nuclei of cells in various mouse embryonic 
      tissues. After treatment of tissue sections with proteinase K, HSP105 but not 
      HSC70 was also found to be localized in condensed cells and condensed bodies 
      which showed the typical characteristics of apoptotic cells and apoptotic bodies 
      at the interdigital regions of limbs. These findings suggest that HSP105 plays 
      important roles during mouse embryo development.
FAU - Hatayama, T
AU  - Hatayama T
AD  - Department of Biochemistry, Kyoto Pharmaceutical University, Japan.
FAU - Takigawa, T
AU  - Takigawa T
FAU - Takeuchi, S
AU  - Takeuchi S
FAU - Shiota, K
AU  - Shiota K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Cell Struct Funct
JT  - Cell structure and function
JID - 7608465
RN  - 0 (HSP110 Heat-Shock Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Hsp105 protein, mouse)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - Embryo, Mammalian/cytology/*metabolism
MH  - Gestational Age
MH  - HSP110 Heat-Shock Proteins
MH  - HSP70 Heat-Shock Proteins/*biosynthesis
MH  - Immunohistochemistry
MH  - Mice
MH  - RNA/isolation & purification
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - 10.1247/csf.22.517 [doi]
PST - ppublish
SO  - Cell Struct Funct. 1997 Oct;22(5):517-25. doi: 10.1247/csf.22.517.