PMID- 9431922
OWN - NLM
STAT- MEDLINE
DCOM- 19980212
LR  - 20200724
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 36
IP  - 1
DP  - 1998 Jan
TI  - Detection of human herpesvirus 6 DNA in serum by a microplate PCR-hybridization 
      assay.
PG  - 68-72
AB  - PCR was performed on DNA extracts derived from clinical serum samples submitted 
      for human herpesvirus 6 (HHV-6) serological examination. To detect amplified 
      HHV-6 products, a hybridization-based microtiter plate assay (PCR ELISA; 
      Boehringer Mannheim) was used. The assay system was found to be rapid, specific, 
      and sensitive. Approximately three copies of a plasmid-based HHV-6 sequence could 
      be detected, and no cross amplification was observed with HHV-7 genomic DNA. 
      There was no correlation found between HHV-6 DNA detection and serological status 
      in clinical serum samples from individuals more than 2 years old. On the other 
      hand, in serum samples from infants less than 2 years old, a high rate of 
      detection of HHV-6 DNA was observed in those who lacked immunoglobulin G and M 
      antibodies to HHV-6 (55%). In this regard, PCR of serum DNA extracts may be used 
      as a sensitive indicator of active HHV-6 infection in infants prior to their 
      seroconversion.
FAU - Osiowy, C
AU  - Osiowy C
AD  - Surveillance, Influenza and Viral Exanthemata, Bureau of Microbiology, Laboratory 
      Centre for Disease Control, Ottawa, Ontario, Canada. carla_osiowy@inet.hwc.ca
FAU - Prud'homme, I
AU  - Prud'homme I
FAU - Monette, M
AU  - Monette M
FAU - Zou, S
AU  - Zou S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Viral/blood
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Herpesvirus 6, Human/*genetics/immunology
MH  - Humans
MH  - Infant
MH  - Middle Aged
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
PMC - PMC124809
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
PMCR- 1998/01/01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
PHST- 1998/01/01 00:00 [pmc-release]
AID - 0475 [pii]
AID - 10.1128/JCM.36.1.68-72.1998 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1998 Jan;36(1):68-72. doi: 10.1128/JCM.36.1.68-72.1998.