PMID- 9433467
OWN - NLM
STAT- MEDLINE
DCOM- 19980130
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 208
IP  - 2
DP  - 1997 Oct 27
TI  - IL-13 induces CD34+ cells isolated from G-CSF mobilized blood to differentiate in 
      vitro into potent antigen presenting cells.
PG  - 117-29
AB  - Dendritic cells (DCs), which are antigen presenting cells of potential use in 
      human antitumor vaccination trials, are presently the subject of intense 
      investigation. Many recent studies have reported the possibility of generating ex 
      vivo large numbers of DCs with high antigen presenting capacity by the culture of 
      bone marrow or blood progenitors. In this study, we examined the differentiation 
      into DCs of CD34+ progenitors isolated from the G-CSF mobilized blood of 3 
      healthy donors and 5 patients with breast cancer and cultured in the presence of 
      GM-CSF + IL-13. The characteristics of the cells were compared to those of cells 
      obtained in the presence of GM-CSF + TNF alpha. By day 15, one third of the bulk 
      cells cultured with IL-13 were CD1a+/CD14- and strongly expressed CD1c, CD40, 
      CD80 and HLA-DR. In contrast, cells obtained with TNF alpha expressed CD1a on one 
      in three cells but with a considerably lower fluorescence intensity than on 
      IL-13-cultured cells and strongly expressed CD14 on more than 50% of cells. 
      CD1a+/CD14- cells emerged in IL-13 cultures at day 5, while in TNF alpha cultures 
      CD14+ cells appeared before CD1a+ cells. Cells grown in the presence of IL-13 had 
      an increased capacity to present antigens to autologous lymphocytes and to 
      stimulate allogeneic T-lymphocytes. This effect was greater than that of cells 
      grown in the presence of TNF alpha. These cells should therefore have greater 
      effector potential in any therapeutic applications in humans.
FAU - Lopez, M
AU  - Lopez M
AD  - Faculte de Medecine, Inserm U76, Paris, France.
FAU - Amorim, L
AU  - Amorim L
FAU - Gane, P
AU  - Gane P
FAU - Cristoph, A
AU  - Cristoph A
FAU - Bardinet, D
AU  - Bardinet D
FAU - Abina, A M
AU  - Abina AM
FAU - Minty, A
AU  - Minty A
FAU - Bernard, J
AU  - Bernard J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigens, CD1)
RN  - 0 (Antigens, CD34)
RN  - 0 (Interleukin-13)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/drug effects/immunology
MH  - Antigens, CD1/immunology
MH  - Antigens, CD34/*immunology
MH  - Cell Cycle
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*drug effects/immunology
MH  - Flow Cytometry
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - Hematopoietic Stem Cells/*cytology/*drug effects/immunology
MH  - Humans
MH  - Interleukin-13/*pharmacology
MH  - Lymphocyte Activation/drug effects/immunology
MH  - Mice
MH  - T-Lymphocytes/drug effects/immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1998/01/20 00:00
MHDA- 1998/01/20 00:01
CRDT- 1998/01/20 00:00
PHST- 1998/01/20 00:00 [pubmed]
PHST- 1998/01/20 00:01 [medline]
PHST- 1998/01/20 00:00 [entrez]
AID - S0022-1759(97)00133-6 [pii]
AID - 10.1016/s0022-1759(97)00133-6 [doi]
PST - ppublish
SO  - J Immunol Methods. 1997 Oct 27;208(2):117-29. doi: 10.1016/s0022-1759(97)00133-6.