PMID- 9433941
OWN - NLM
STAT- MEDLINE
DCOM- 19980226
LR  - 20081121
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 27
IP  - 12
DP  - 1997 Dec
TI  - A crossreactivity at the immunoglobulin E level of the cell wall mannoproteins of 
      Candida albicans with other pathogenic Candida and airborne yeast species.
PG  - 1449-57
AB  - BACKGROUND: Candida albicans crossreacts with Saccharomyces cerevisiae or 
      Pityrosporum ovale at the IgE level. However, the extent of crossreactivity of C. 
      albicans with other yeast species is not known. OBJECTIVE: The crossreactivity at 
      the immunoglobulin E (IgE) level of Candida albicans with other pathogenic 
      Candida species and to the airborne yeast species Cryptococcus and Rhodotorula 
      was studied by immunoblot analysis. METHODS: Crude antigens, designated as heat 
      extract, were prepared from 13 different yeast species and a dot blot test was 
      performed to detect IgE antibodies against each of the heat extracts in 349 
      patients with allergies who were positive for IgE antibodies against C. albicans 
      in a CAP system. RESULTS: In the dot blot test, most of the sera reacted with the 
      heat extracts of not only C. albicans but also those prepared from the other 
      yeast species. The sera of 41 of the 349 patients (11.7%) reacted with the heat 
      extracts of all 13 yeast species. The extent of the binding of IgE antibodies to 
      multiple yeast species correlated with both the fluorescence intensities measured 
      in the CAP system and the intensities of dots generated by the heat extract of C. 
      albicans in the dot blot test. In an inhibition dot blot test, mannoproteins, but 
      not proteins, of C. albicans strongly inhibited the subsequent binding of IgE 
      antibodies to all yeast species. CONCLUSION: Our data suggest that the C. 
      albicans mannoproteins are responsible for the crossreactivity among these yeast 
      species at the IgE level.
FAU - Kanbe, T
AU  - Kanbe T
AD  - Laboratory of Medical Mycology, Research Institute for Disease Mechanism and 
      Control, Nagoya University School of Medicine, Japan.
FAU - Utsunomiya, K
AU  - Utsunomiya K
FAU - Ishiguro, A
AU  - Ishiguro A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Air Pollutants)
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cell Extracts)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Rosaniline Dyes)
RN  - 0 (mannoproteins)
RN  - 11028-71-0 (Concanavalin A)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 78642-64-5 (Coomassie blue)
SB  - IM
MH  - *Air Microbiology
MH  - Air Pollutants/*immunology
MH  - Allergens/adverse effects
MH  - Antibodies, Fungal/immunology/metabolism
MH  - Antibody Specificity
MH  - Antigens, Fungal/immunology
MH  - Candida/*immunology
MH  - Candida albicans/*immunology
MH  - Cell Extracts/analysis/immunology
MH  - Cell Wall/chemistry/immunology
MH  - Concanavalin A
MH  - Cross Reactions/immunology
MH  - Cryptococcus/immunology
MH  - Hot Temperature
MH  - Humans
MH  - Hypersensitivity/blood/etiology/microbiology
MH  - Immunoblotting
MH  - Immunoglobulin E/blood/immunology/*metabolism
MH  - Membrane Glycoproteins/adverse effects/*immunology/metabolism
MH  - Protein Binding
MH  - Rhodotorula/immunology
MH  - Rosaniline Dyes
MH  - Species Specificity
MH  - Staining and Labeling
MH  - Yeasts/*immunology
EDAT- 1998/01/20 00:00
MHDA- 1998/01/20 00:01
CRDT- 1998/01/20 00:00
PHST- 1998/01/20 00:00 [pubmed]
PHST- 1998/01/20 00:01 [medline]
PHST- 1998/01/20 00:00 [entrez]
PST - ppublish
SO  - Clin Exp Allergy. 1997 Dec;27(12):1449-57.