PMID- 9434179
OWN - NLM
STAT- MEDLINE
DCOM- 19980212
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 204
IP  - 1-2
DP  - 1997 Dec 19
TI  - Cloning and characterization of the human Calmegin gene encoding putative 
      testis-specific chaperone.
PG  - 159-63
AB  - The putative chaperone Calmegin is required for sperm fertility in mouse and the 
      relevance of the gene to certain cases of human male infertility has been 
      suggested. In the present paper, we have isolated and characterized the human 
      homolog cDNA of the mouse germ cell-specific Calmegin. The entire coding region 
      of the human cDNA showed 80% identity with the previously reported mouse 
      Calmegin. The predicted amino acid sequence showed strong conservation of the two 
      sets of internal repetitive sequences (Ca2+ binding motif), and the hydrophilic 
      COOH terminus, which corresponds to the putative endoplasmic reticulum (ER) 
      retention motif. Our finding will support diagnosis of male infertility. Northern 
      blotting analysis of various human tissues showed that the transcript was 3 kb in 
      length and was expressed exclusively in the testis. Using the fluorescence in 
      situ hybridization (FISH) technique, human Calmegin gene was mapped to chromosome 
      4q28.3-q31.1.
FAU - Tanaka, H
AU  - Tanaka H
AD  - Department of Science for Laboratory Animal Experimentation, Research Institute 
      for Microbial Diseases, Osaka University, Suita City, Japan.
FAU - Ikawa, M
AU  - Ikawa M
FAU - Tsuchida, J
AU  - Tsuchida J
FAU - Nozaki, M
AU  - Nozaki M
FAU - Suzuki, M
AU  - Suzuki M
FAU - Fujiwara, T
AU  - Fujiwara T
FAU - Okabe, M
AU  - Okabe M
FAU - Nishimune, Y
AU  - Nishimune Y
LA  - eng
SI  - GENBANK/D86322
SI  - GENBANK/D86323
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Molecular Chaperones)
RN  - 139873-08-8 (Calnexin)
RN  - 155982-67-5 (calmegin)
RN  - EC 3.6.1.- (Chaperonins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Calcium-Binding Proteins/*genetics/metabolism
MH  - *Calnexin
MH  - Chaperonins/*genetics/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 4
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Metaphase
MH  - Mice
MH  - Molecular Chaperones
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Testis/*metabolism
EDAT- 1998/01/20 00:00
MHDA- 1998/01/20 00:01
CRDT- 1998/01/20 00:00
PHST- 1998/01/20 00:00 [pubmed]
PHST- 1998/01/20 00:01 [medline]
PHST- 1998/01/20 00:00 [entrez]
AID - S0378-1119(97)00537-4 [pii]
AID - 10.1016/s0378-1119(97)00537-4 [doi]
PST - ppublish
SO  - Gene. 1997 Dec 19;204(1-2):159-63. doi: 10.1016/s0378-1119(97)00537-4.