PMID- 9434799
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20190822
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 86
IP  - 1
DP  - 1998 Jan
TI  - Autoantibodies to a novel early endosome antigen 1.
PG  - 81-7
AB  - Autoantibodies to EEA1, an antigen on early endosomes, were first reported in the 
      serum of a patient with subacute cutaneous lupus erythematosus (SCLE). Here we 
      have examined 38 sera selected for investigation of autoantibodies to EEA1 on the 
      basis of cytoplasmic vesicle-like reactivity by immunofluorescence. Ten of the 
      sera were reactive to a HeLa cell protein of approximately the same M(r) as human 
      EEA1. Eight of these sera belonged to the IgG1 subclass. Five of the sera reacted 
      with fusion proteins incorporating either the amino (from amino acids 1 to 209) 
      or the carboxyl (incorporating the most C-terminal 300 amino acids) terminus of 
      the human EEA1 protein. Antigens reactive with these 5 sera colocalized with 
      internalized transferrin receptors, indicating their association with early 
      endosomes. The other 5 sera which did not react to both fusion proteins did not 
      colocalize with internalized transferrin receptors. We conclude that 5 of the 38 
      patients (13%) have autoantibodies to EEA1. None of these patients have SCLE, but 
      have generalized joint pain, polyarthritis, rheumatoid arthritis, or circulating 
      rheumatoid factors.
FAU - Waite, R L
AU  - Waite RL
AD  - Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, 
      Australia.
FAU - Sentry, J W
AU  - Sentry JW
FAU - Stenmark, H
AU  - Stenmark H
FAU - Toh, B H
AU  - Toh BH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (early endosome antigen 1)
RN  - 9009-79-4 (Rheumatoid Factor)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibody Specificity
MH  - Arthralgia/blood/immunology
MH  - Arthritis/blood/immunology
MH  - Arthritis, Rheumatoid/blood/immunology
MH  - Autoantibodies/blood/*immunology
MH  - Autoimmune Diseases/blood/*immunology
MH  - Biomarkers
MH  - Endosomes/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - HeLa Cells
MH  - Humans
MH  - Immunoglobulin G/blood/immunology
MH  - Immunoglobulin M/blood/immunology
MH  - Joint Diseases/blood/*immunology
MH  - Male
MH  - Membrane Proteins/*immunology
MH  - Receptors, Transferrin/analysis
MH  - Recombinant Fusion Proteins/immunology
MH  - Rheumatoid Factor/analysis
MH  - Vesicular Transport Proteins
EDAT- 1998/01/22 00:00
MHDA- 1998/01/22 00:01
CRDT- 1998/01/22 00:00
PHST- 1998/01/22 00:00 [pubmed]
PHST- 1998/01/22 00:01 [medline]
PHST- 1998/01/22 00:00 [entrez]
AID - S009012299794455X [pii]
AID - 10.1006/clin.1997.4455 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1998 Jan;86(1):81-7. doi: 10.1006/clin.1997.4455.