PMID- 94356
OWN - NLM
STAT- MEDLINE
DCOM- 19800616
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 32
IP  - 1
DP  - 1979 Oct
TI  - Mice with spontaneous mammary tumors develop type-specific neutralizing and 
      cytotoxic antibodies against the mouse mammary tumor virus envelope protein gp52.
PG  - 131-9
AB  - Sera from C3H mammary tumor-bearing mice contain cytotoxic antibodies for mouse 
      mammary tumor virus (MMTV)-producing cells, based on (51)Cr release in a 
      complement-dependent serum cytotoxicity assay. The cytotoxic antibodies could be 
      absorbed by purified C3H MMTV gp52 and C3H MMTV-infected cat cells (C3H [MMTV] 
      CrFK) containing cell surface MMTV gp52. However, purified MMTV p27 and 
      uninfected CrFK cat cells were negative. Absorption of the sera with GR (MMTV) 
      CrFK cells also removed all of the cytotoxicity, whereas absorption with RIII 
      (MMTV) CrFK cells was negative, even though all three infected cat cells 
      contained equivalent amounts of gp52. The same C3H cytotoxic sera also 
      neutralized the focus-forming capacity of a C3H MMTV pseudotype of Kirsten 
      sarcoma virus containing MMTV gp52. In contrast, sera from mammary tumor-bearing 
      GR and RIII mice did not neutralize the pseudotype. Furthermore, neutralization 
      could be achieved only by anti-gp52 but not by anti-gp36, -p27, -p14, or -p10 C3H 
      MMTV sera. The gp52's of C3H, GR, and RIII MMTV could also be distinguished by 
      using a type-specific competition radioimmunoassay employing (125)I-gp52 of C3H 
      MMTV and a hyperimmune rabbit anti-C3H MMTV serum. To demonstrate these 
      differences directly, we studied the primary structure of gp52 on the surface of 
      the C3H, GR, and RIII (MMTV) CrFK cells. Two-dimensional tryptic peptide maps of 
      the cell surface lactoper-oxidase-catalyzed iodinated gp52's revealed a greater 
      number of peptides common to the gp52's of C3H and GR MMTVs than to RIII MMTV 
      gp52. These results demonstrate that gp52 is a major target antigen for both 
      cytotoxic and neutralizing antibodies, that the cell surface and 
      virion-associated gp52's of C3H, GR, and RIII MMTV contain both group- and 
      type-specific determinants, and that C3H and GR MMTV gp52's are antigenically 
      more related to each other than to RIII MMTV gp52. Furthermore, C3H mammary 
      tumor-bearing mice develop type-specific antibodies capable of recognizing unique 
      gp52 determinants and, therefore, are able to distinguish the gp52 of C3H MMTV 
      from the gp52's of GR and RIII MMTV.
FAU - Schochetman, G
AU  - Schochetman G
FAU - Arthur, L O
AU  - Arthur LO
FAU - Long, C W
AU  - Long CW
FAU - Massey, R J
AU  - Massey RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Surface)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*biosynthesis
MH  - Antigens, Surface/*immunology
MH  - Cell Line
MH  - Cytotoxicity, Immunologic
MH  - Epitopes
MH  - Female
MH  - Glycoproteins/analysis/*immunology
MH  - Mammary Neoplasms, Experimental/*immunology
MH  - Mammary Tumor Virus, Mouse/analysis/*immunology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Neutralization Tests
MH  - Peptides/analysis
MH  - Radioimmunoassay
MH  - Viral Proteins/analysis/*immunology
PMC - PMC353535
EDAT- 1979/10/01 00:00
MHDA- 1979/10/01 00:01
PMCR- 1979/10/01
CRDT- 1979/10/01 00:00
PHST- 1979/10/01 00:00 [pubmed]
PHST- 1979/10/01 00:01 [medline]
PHST- 1979/10/01 00:00 [entrez]
PHST- 1979/10/01 00:00 [pmc-release]
AID - 10.1128/JVI.32.1.131-139.1979 [doi]
PST - ppublish
SO  - J Virol. 1979 Oct;32(1):131-9. doi: 10.1128/JVI.32.1.131-139.1979.