PMID- 9437853
OWN - NLM
STAT- MEDLINE
DCOM- 19980212
LR  - 20190921
IS  - 0022-104X (Print)
IS  - 0022-104X (Linking)
VI  - 280
IP  - 1
DP  - 1998 Jan 1
TI  - Identification of sperm-bearing female-specific chromosome in the sex-reversed 
      chicken.
PG  - 65-72
AB  - Sexual differentiation in the female chick embryo was phenotypically reversed to 
      the male sex by injection of an aromatase inhibitor (Fadrozole, 0.1 mg/egg) into 
      the embryo at day 5 of incubation. The birds were raised to 10 months of age, and 
      the morphology of the gonads of sex-reversed hens were examined by the 
      light-microscopic morphology, and the presence of the W chromosome gene was 
      determined by fluorescence in situ hybridization (FISH) technique and used for 
      PCR analysis of a single isolated sperm. The sex-reversed hens possessed two 
      testes with a fully developed oviduct on the left side. The testes contained 
      essentially the same cellular components as those of normal testes, although 
      sperm counts were low. FISH analysis revealed numerous spermatids and several 
      sperm bearing W-chromosomes, indicating that the second meiosis occurred normally 
      but that the transformation from the spermatid to the spermatozoon is partially 
      impaired. PCR analysis using the DNA of a single sperm also indicates that 
      sperm-carrying the W chromosome were produced.
FAU - Abinawanto
AU  - Abinawanto
AD  - Laboratory of Animal Physiology, School of Agricultural Sciences, Nagoya 
      University, Japan.
FAU - Zhang, C
AU  - Zhang C
FAU - Saito, N
AU  - Saito N
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Shimada, K
AU  - Shimada K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Exp Zool
JT  - The Journal of experimental zoology
JID - 0375365
RN  - 0 (Aromatase Inhibitors)
RN  - 9007-49-2 (DNA)
RN  - H3988M64PU (Fadrozole)
SB  - IM
MH  - Animals
MH  - Aromatase Inhibitors
MH  - Base Sequence
MH  - Chickens/genetics/*physiology
MH  - DNA/analysis/chemistry/genetics
MH  - *Disorders of Sex Development
MH  - Fadrozole/administration & dosage/pharmacology
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Injections/veterinary
MH  - Male
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - *Sex Chromosomes
MH  - Sex Differentiation/genetics/physiology
MH  - Spermatids/cytology/physiology
MH  - Spermatozoa/chemistry/cytology/*physiology
MH  - Testis/cytology
EDAT- 1998/01/23 06:09
MHDA- 2000/06/20 09:00
CRDT- 1998/01/23 06:09
PHST- 1998/01/23 06:09 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/01/23 06:09 [entrez]
AID - 10.1002/(SICI)1097-010X(19980101)280:1<65::AID-JEZ8>3.0.CO;2-F [pii]
AID - 10.1002/(sici)1097-010x(19980101)280:1<65::aid-jez8>3.0.co;2-f [doi]
PST - ppublish
SO  - J Exp Zool. 1998 Jan 1;280(1):65-72. doi: 
      10.1002/(sici)1097-010x(19980101)280:1<65::aid-jez8>3.0.co;2-f.