PMID- 9438344
OWN - NLM
STAT- MEDLINE
DCOM- 19980317
LR  - 20190831
IS  - 0015-5632 (Print)
IS  - 0015-5632 (Linking)
VI  - 42
IP  - 5
DP  - 1997
TI  - Polyketide synthase gene pksM from Aspergillus terreus expressed during growth 
      phase.
PG  - 419-30
AB  - The polyketide synthase gene pksM was detected in the genomic DNA library of 
      Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase 
      (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were 
      sequenced and a 5.5 kb open reading frame was revealed. A single intron (62 bp) 
      was identified in the conserved position. Two transcription start points were 
      determined within the 5'-flanking region at 50 and 72 (major) bp upstream from 
      the putative translation initiation codon ATG. The conserved active site motifs 
      for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier 
      protein were found within the predicted polypeptide consisting of 1803 amino 
      acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. 
      terreus was observed in the middle of the vegetative growth phase.
FAU - Pazoutova, S
AU  - Pazoutova S
AD  - Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, 
      Czech Republic.
FAU - Linka, M
AU  - Linka M
FAU - Storkova, S
AU  - Storkova S
FAU - Schwab, H
AU  - Schwab H
LA  - eng
SI  - GENBANK/U31329
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Folia Microbiol (Praha)
JT  - Folia microbiologica
JID - 0376757
RN  - 0 (Acyl Carrier Protein)
RN  - 0 (Codon, Initiator)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Fungal)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (RNA, Fungal)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.100 (3-Oxoacyl-(Acyl-Carrier-Protein) Reductase)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.41 (3-Oxoacyl-(Acyl-Carrier-Protein) Synthase)
RN  - EC 4.2.1.- (Hydro-Lyases)
RN  - EC 6.- (6-methylsalicylic acid synthetase)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
MH  - 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics
MH  - Acyl Carrier Protein/genetics
MH  - Acyltransferases/genetics
MH  - Alcohol Oxidoreductases/genetics
MH  - Amino Acid Sequence
MH  - Aspergillus/*genetics/growth & development/*metabolism
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - Codon, Initiator
MH  - DNA Probes
MH  - DNA, Fungal/analysis/genetics
MH  - Gene Expression
MH  - Gene Library
MH  - Genes, Fungal
MH  - Hydro-Lyases/genetics
MH  - Introns
MH  - Ligases/genetics
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/*genetics/*metabolism
MH  - Nucleic Acid Hybridization
MH  - Open Reading Frames
MH  - Oxidoreductases/genetics
MH  - Penicillium/genetics
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - RNA, Fungal/isolation & purification
MH  - Restriction Mapping
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
EDAT- 1997/01/01 00:00
MHDA- 1998/01/23 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/01/23 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/BF02826548 [doi]
PST - ppublish
SO  - Folia Microbiol (Praha). 1997;42(5):419-30. doi: 10.1007/BF02826548.