PMID- 9438814
OWN - NLM
STAT- MEDLINE
DCOM- 19980318
LR  - 20111117
IS  - 0895-0385 (Print)
IS  - 0895-0385 (Linking)
VI  - 10
IP  - 6
DP  - 1997 Dec
TI  - Matrix metalloproteinase-3 production by human degenerated intervertebral disc.
PG  - 493-8
AB  - To assess its possible role in the pathophysiology of intervertebral disc 
      degeneration, we investigated the production of matrix metalloproteinase-3 
      (MMP-3) using human intervertebral disc explant culture. Five normal and 10 
      degenerated disc specimens were used. The levels of MMP-3 released in the medium 
      were measured with use of an enzyme immunoassay. The results showed that the 
      level of MMP-3 in the degenerated group (0.57 microg/ml/mg wet weight; n = 10) 
      was significantly higher than that of the control group (0.29 microg/ml/mg wet 
      weight; n = 5) (p < 0.05). Immunostaining of MMP-3 revealed that the ratio of 
      positive staining cells in the degenerated group was greater than that of the 
      control group. These observations suggest that MMP-3 produced by human 
      intervertebral disc may be involved in the intervertebral disc degeneration, 
      particularly in the initiation of matrix degradation of intervertebral disc.
FAU - Nemoto, O
AU  - Nemoto O
AD  - Department of Orthopaedic Surgery, National Defense Medical College, Tokorozawa, 
      Saitama, Japan.
FAU - Yamagishi, M
AU  - Yamagishi M
FAU - Yamada, H
AU  - Yamada H
FAU - Kikuchi, T
AU  - Kikuchi T
FAU - Takaishi, H
AU  - Takaishi H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Spinal Disord
JT  - Journal of spinal disorders
JID - 8904842
RN  - 0 (Culture Media, Conditioned)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Culture Media, Conditioned/chemistry
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Humans
MH  - Intervertebral Disc/*enzymology
MH  - Intervertebral Disc Displacement/enzymology
MH  - Male
MH  - Matrix Metalloproteinase 3/*biosynthesis
MH  - Middle Aged
MH  - Organ Culture Techniques
MH  - Spinal Diseases/*enzymology
MH  - Spondylolisthesis/enzymology
EDAT- 1998/01/23 00:00
MHDA- 1998/01/23 00:01
CRDT- 1998/01/23 00:00
PHST- 1998/01/23 00:00 [pubmed]
PHST- 1998/01/23 00:01 [medline]
PHST- 1998/01/23 00:00 [entrez]
PST - ppublish
SO  - J Spinal Disord. 1997 Dec;10(6):493-8.