PMID- 9441852
OWN - NLM
STAT- MEDLINE
DCOM- 19980303
LR  - 20071114
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 13
IP  - 3
DP  - 1997 Nov
TI  - Analysis of DNA replication by fluorescence in situ hybridization.
PG  - 259-70
AB  - Fluorescence in situ hybridization (FISH) has been shown to discriminate between 
      unreplicated and replicated regions of the genome in interphase nuclei, based on 
      the number of specific fluorescent signals that can be detected. By examining the 
      replication status of hybridizing sequences in large numbers of individual cells 
      from an asynchronously growing population, it is possible to deduce a relative 
      order of replication of different sequences. The availability of well-mapped 
      genomic probes and the ability to compare results from different cell lines make 
      this a convenient approach with which to map domains of replication timing 
      control at any chromosomal position and to relate this to various patterns of 
      gene expression. Since there appear to be important but poorly understood 
      correlations among replication timing, chromatin structure, and transcriptional 
      competence in mammalian cells, this provides a valuable approach to understanding 
      these interrelationships at the molecular level. The procedures for using FISH to 
      examine replication timing in mammalian nuclei are described here in detail, and 
      the advantages and limitations of the approach are discussed. Some other 
      strategies for using high-resolution FISH on chromatin fibers to examine 
      replication properties of specific sequences in situ are also described.
FAU - Boggs, B A
AU  - Boggs BA
AD  - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, 
      Texas 77030, USA.
FAU - Chinault, A C
AU  - Chinault AC
LA  - eng
GR  - GM53033/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
SB  - IM
MH  - Animals
MH  - *DNA Replication
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
RF  - 55
EDAT- 1998/01/27 00:00
MHDA- 1998/01/27 00:01
CRDT- 1998/01/27 00:00
PHST- 1998/01/27 00:00 [pubmed]
PHST- 1998/01/27 00:01 [medline]
PHST- 1998/01/27 00:00 [entrez]
AID - S1046-2023(97)90525-9 [pii]
AID - 10.1006/meth.1997.0525 [doi]
PST - ppublish
SO  - Methods. 1997 Nov;13(3):259-70. doi: 10.1006/meth.1997.0525.