PMID- 9441901
OWN - NLM
STAT- MEDLINE
DCOM- 19980831
LR  - 20161020
IS  - 1089-8603 (Print)
IS  - 1089-8603 (Linking)
VI  - 1
IP  - 4
DP  - 1997 Aug
TI  - An unprocessed pseudogene of inducible nitric oxide synthase gene in human.
PG  - 294-300
AB  - Inducible nitric oxide synthase (iNOS or NOSII) is one of three distinct NOS 
      isoforms in human. The NOSII isoform is expressed in a variety of cells and 
      tissues in response to endotoxins and cytokines. The human genome contains at 
      least two loci for the NOSII gene, one of which (NOSII-1) has previously been 
      assigned to proximal region of the long arm (cen-q11.2 or q11.2-q12) or to 
      pericentric (p11-q11) regions of chromosome 17. The present study, carried out 
      using fluorescence in situ hybridization (FISH) method, shows that a pseudogene 
      gene (NOSII-2) is mapped to chromosome 17q11.2 site. The NOSII-2 sequence 
      contains the exon and intron sequences present in NOSII-1 but with several 
      mutations such as single base substitutions, additions, and deletions. 
      Additionally, the NOSII-2 sequence also contains an incomplete reductase domain 
      which corresponds only to the cofactor binding sites without the oxygenase domain 
      that carry heme and substrate binding sites. NOSII-2, therefore, appears to be an 
      unprocessed pseudogene, which cannot be translated to a functional enzyme because 
      of its incomplete sequences and mutations.
FAU - Park, C S
AU  - Park CS
AD  - Section of Chemical Pharmacology, Laboratory of Molecular Immunology, NHLBI, 
      National Institutes of Health, Bethesda, Maryland 20892, USA. 
      parkshin@dragon.inha.ac.kr
FAU - Lee, H S
AU  - Lee HS
FAU - Lee, H Y
AU  - Lee HY
FAU - Krishna, G
AU  - Krishna G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Nitric Oxide
JT  - Nitric oxide : biology and chemistry
JID - 9709307
RN  - 0 (DNA, Complementary)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 17
MH  - DNA, Complementary
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nitric Oxide Synthase/*genetics
MH  - Nitric Oxide Synthase Type II
MH  - *Pseudogenes
EDAT- 1997/08/01 00:00
MHDA- 1998/01/27 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1998/01/27 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S1089-8603(97)90138-5 [pii]
AID - 10.1006/niox.1997.0138 [doi]
PST - ppublish
SO  - Nitric Oxide. 1997 Aug;1(4):294-300. doi: 10.1006/niox.1997.0138.