PMID- 9446660
OWN - NLM
STAT- MEDLINE
DCOM- 19980218
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 91
IP  - 3
DP  - 1998 Feb 1
TI  - Application of interphase fluorescence in situ Hybridization for the detection of 
      the Burkitt translocation t(8;14)(q24;q32) in B-cell lymphomas.
PG  - 984-90
AB  - The translocation t(8;14)(q24;q32) is the characteristic chromosomal aberration 
      of Burkitt's-type lymphomas and leukemias (BLs). On the molecular level, the 
      t(8;14) juxtaposes the c-myc gene in 8q24 next to the IgH locus in 14q32, 
      resulting in overexpression of the transcription factor c-Myc. The detection of a 
      t(8;14) is a major aim in the diagnostic process of all patients with high-grade 
      B-cell lymphomas because treatment strategies differ between BL and other 
      high-grade lymphomas. As chromosome analyses are sometimes hampered by the low 
      yield or poor quality of metaphase spreads and as the application of molecular 
      genetic techniques is limited by the distribution of the 8q24 breakpoints over a 
      region of about some hundred kilobases, we set out to establish an interphase 
      fluorescence in situ hybridization (FISH) assay for the detection of the t(8;14). 
      A cosmid probe hybridizing to the IgH constant region in 14q32 was combined with 
      a differently labeled probe of pooled cosmid clones spanning the c-myc locus in 
      8q24. Interphase nuclei lacking a t(8;14) show two separated signals 
      corresponding to each probe, whereas interphase nuclei carrying a t(8;14) display 
      a split of the c-myc probe and a colocalization of at least one of the splitted 
      signals with the IgH probe. Based on the results of extensive control studies, 
      the cutoff level for this stringent (type I) criteria was set at 2%. 
      Additionally, colocalization of at least one c-myc signal with one IgH signal 
      alone (without signal split for the c-myc probe) was used as a less stringent 
      (type II) criteria with a cutoff limit of 11%. Nine BLs and one Burkitt-like 
      lymphoma were investigated by this approach. Cytogenetically, all tumors 
      contained a translocation t(8;14)(q24;q32) except for one BL, in which 
      cytogenetic analysis had failed. In interphase FISH, all lymphomas and leukemias 
      met the less stringent criteria for the diagnosis of the t(8;14). Additionally, 
      in all tumors but the Burkitt-like lymphoma, a t(8;14) could be diagnosed 
      according to the stringent criteria. The percentage of cells found to harbor the 
      t(8;14) by FISH ranged from 4.3% to 100%. Comparison of cytogenetic and FISH 
      results revealed a significantly lower percentage of t(8;14)+ interphase nuclei 
      than metaphase cells (P = .004). In conclusion, the described FISH assay provides 
      a feasible and sensitive tool for the routine detection of the translocation 
      t(8;14) in interphase cells which might also offer new insights into the biology 
      of high-grade B-cell lymphomas.
FAU - Siebert, R
AU  - Siebert R
AD  - Department of Human Genetics, University of Kiel, Germany.
FAU - Matthiesen, P
AU  - Matthiesen P
FAU - Harder, S
AU  - Harder S
FAU - Zhang, Y
AU  - Zhang Y
FAU - Borowski, A
AU  - Borowski A
FAU - Zuhlke-Jenisch, R
AU  - Zuhlke-Jenisch R
FAU - Metzke, S
AU  - Metzke S
FAU - Joos, S
AU  - Joos S
FAU - Weber-Matthiesen, K
AU  - Weber-Matthiesen K
FAU - Grote, W
AU  - Grote W
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Adult
MH  - Burkitt Lymphoma/*genetics
MH  - Child
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 1998/02/03 00:00
MHDA- 1998/02/03 00:01
CRDT- 1998/02/03 00:00
PHST- 1998/02/03 00:00 [pubmed]
PHST- 1998/02/03 00:01 [medline]
PHST- 1998/02/03 00:00 [entrez]
AID - S0006-4971(20)55356-1 [pii]
PST - ppublish
SO  - Blood. 1998 Feb 1;91(3):984-90.