PMID- 9446783
OWN - NLM
STAT- MEDLINE
DCOM- 19980227
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 242
IP  - 2
DP  - 1998 Jan 14
TI  - 2-2'-Pyridylisatogen, a selective allosteric modulator of P2 receptors, is a spin 
      trapping agent.
PG  - 272-6
AB  - 2-2'-Pyridylisatogen (PIT) has been reported to be a relatively selective 
      irreversible antagonist of responses to adenosine 5'-triphosphate (ATP) in some 
      smooth muscle preparations and to be an allosteric modulator of responses to ATP 
      at recombinant P2Y receptors from chick brain. PIT is also a potent inhibitor of 
      mitochondrial oxidative phosphorylation. However, the compound has a unique 
      nitrone structure, so PIT was compared with dimethyl-pyrroline-N-oxide (DMPO) as 
      a spin trapping agent for superoxide and hydroxyl radicals using electron spin 
      resonance (ESR). PIT was found to be a potent spin trapper of both hydroxyl and 
      superoxide radicals. PIT was more potent than DMPO to trap the hydroxyl radical 
      forming an adduct which was more stable than the DMPO adduct in aqueous media. 
      PIT was an effective spin trap of hydroxyl radical in aqueous buffer at pH 7.4. 
      PIT more slowly trapped the superoxide anion but at concentrations where DMPO 
      trapped none.
FAU - Nepveu, F
AU  - Nepveu F
AD  - Universite Paul Sabatier, Faculte des Science Pharmaceutiques, Toulouse, France.
FAU - Souchard, J P
AU  - Souchard JP
FAU - Rolland, Y
AU  - Rolland Y
FAU - Dorey, G
AU  - Dorey G
FAU - Spedding, M
AU  - Spedding M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cyclic N-Oxides)
RN  - 0 (Fenton's reagent)
RN  - 0 (Nitrogen Oxides)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Spin Labels)
RN  - 11062-77-4 (Superoxides)
RN  - 1AVZ07U9S7 (Xanthine)
RN  - 2922-11-4 (2,2'-pyridylisatogen)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 3I91332OPG (phenyl-N-tert-butylnitrone)
RN  - 7170JZ1QF3 (5,5-dimethyl-1-pyrroline-1-oxide)
RN  - 82X95S7M06 (Isatin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.17.3.2 (Xanthine Oxidase)
SB  - IM
MH  - Allosteric Regulation/physiology
MH  - Cyclic N-Oxides/metabolism
MH  - Electron Spin Resonance Spectroscopy
MH  - Hydrogen Peroxide/metabolism
MH  - Hydroxyl Radical/analysis/metabolism
MH  - Iron/metabolism
MH  - Isatin/*analogs & derivatives/pharmacology
MH  - Kinetics
MH  - Molecular Structure
MH  - Nitrogen Oxides/metabolism
MH  - Receptors, Purinergic P2/*metabolism
MH  - *Spin Labels
MH  - Spin Trapping
MH  - Superoxides/analysis/metabolism
MH  - Xanthine/metabolism
MH  - Xanthine Oxidase/metabolism
EDAT- 1998/02/03 00:00
MHDA- 1998/02/03 00:01
CRDT- 1998/02/03 00:00
PHST- 1998/02/03 00:00 [pubmed]
PHST- 1998/02/03 00:01 [medline]
PHST- 1998/02/03 00:00 [entrez]
AID - S0006-291X(97)97949-3 [pii]
AID - 10.1006/bbrc.1997.7949 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Jan 14;242(2):272-6. doi: 
      10.1006/bbrc.1997.7949.