PMID- 9447825
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 12
DP  - 1997 Dec
TI  - Detection of CBP rearrangements in acute myelogenous leukemia with t(8;16).
PG  - 2087-96
AB  - The CREB-binding protein (CBP) is a large nuclear protein that regulates many 
      signal transduction pathways and is involved in chromatin-mediated transcription. 
      The translocation t(8;16)(p11;p13.3) consistently disrupts two genes: the CBP 
      gene on chromosome band 16p13.3 and the MOZ gene on chromosome band 8p11. 
      Although a fusion of these two genes as a result of the translocation is 
      expected, attempts at detecting the fusion transcript by reverse transcriptase 
      polymerase chain reaction (RT-PCR) have proven difficult; to date, only one 
      in-frame CBP/MOZ fusion transcript has been reported. We therefore sought other 
      reliable means of detecting CBP rearrangements. We applied fluorescence in situ 
      hybridization (FISH) and Southern blot analyses to a series of AML patients with 
      a t(8;16) and detected DNA rearrangements of both the CBP and the MOZ loci in all 
      cases tested. All six cases examined for CBP rearrangements have breakpoints 
      within a 13 kb breakpoint cluster region at the 5' end of the CBP gene. 
      Additionally, we used a MOZ cDNA probe to construct a surrounding cosmid contig 
      and detect DNA rearrangements in three t(8;16) cases, all of which display 
      rearrangements within a 6 kb genomic fragment of the MOZ gene. We have thus 
      developed a series of cosmid probes that consistently detect the disruption of 
      the CBP gene in t(8;16) patients. These clones could potentially be used to 
      screen other cancer-associated or congenital translocations involving chromosome 
      band 16p13.3 as well.
FAU - Giles, R H
AU  - Giles RH
AD  - Department of Human Genetics, Leiden University, The Netherlands.
FAU - Dauwerse, J G
AU  - Dauwerse JG
FAU - Higgins, C
AU  - Higgins C
FAU - Petrij, F
AU  - Petrij F
FAU - Wessels, J W
AU  - Wessels JW
FAU - Beverstock, G C
AU  - Beverstock GC
FAU - Dohner, H
AU  - Dohner H
FAU - Jotterand-Bellomo, M
AU  - Jotterand-Bellomo M
FAU - Falkenburg, J H
AU  - Falkenburg JH
FAU - Slater, R M
AU  - Slater RM
FAU - van Ommen, G J
AU  - van Ommen GJ
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - van der Reijden, B A
AU  - van der Reijden BA
FAU - Breuning, M H
AU  - Breuning MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (CREBBP protein, human)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (KAT6A protein, human)
SB  - IM
MH  - Acetyltransferases/genetics
MH  - Blotting, Southern
MH  - CREB-Binding Protein
MH  - *Chromosomes, Human, Pair 16
MH  - *Chromosomes, Human, Pair 8
MH  - *Gene Rearrangement
MH  - Histone Acetyltransferases
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Nuclear Proteins/*genetics
MH  - *Trans-Activators
MH  - Transcription Factors/*genetics
MH  - *Translocation, Genetic
EDAT- 1998/02/03 00:00
MHDA- 1998/02/03 00:01
CRDT- 1998/02/03 00:00
PHST- 1998/02/03 00:00 [pubmed]
PHST- 1998/02/03 00:01 [medline]
PHST- 1998/02/03 00:00 [entrez]
AID - 10.1038/sj.leu.2400882 [doi]
PST - ppublish
SO  - Leukemia. 1997 Dec;11(12):2087-96. doi: 10.1038/sj.leu.2400882.