PMID- 9447827
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 12
DP  - 1997 Dec
TI  - Increased frequency of chromosome abnormalities in fibroblasts from hairy cell 
      leukemia patients.
PG  - 2105-10
AB  - A hereditary component is implicated in many different cancers, including hairy 
      cell leukemia (HCL), and may involve an instability of the genome. We have 
      previously documented recurrent clonal and non-clonal chromosomal abnormalities 
      in hairy cells. To ascertain whether this instability of the genome is restricted 
      to the malignant cells or if it might also include normal cells we performed 
      cytogenetic investigations on skin fibroblasts and hairy cells from eight HCL 
      patients and skin fibroblasts from eight referents. The frequency of chromosome 
      abnormalities, regardless of clonality, was significantly increased in the 
      fibroblasts from patients compared to referents. Also, five patients compared to 
      one referent showed clonal abnormalities in their fibroblasts. 
      Immunohistochemical investigations excluded the possibility that the fibroblast 
      cultures were contaminated with hairy cells. Two patients had constitutional 
      abnormalities, inv(5)(p13.1q13.3) and t(13;14), and one additional patient, 
      possibly mosaic, showed the same abnormality, inv(9)(p21-22q22), in both 
      fibroblasts (17/30) and blood (5/21) cells. Aberrations in patient fibroblasts 
      also included sporadic inv(5), del(6)q, inv(19), and del(20)q, abnormalities 
      previously shown to occur in hairy cells. A clonal expansion with trisomy 7 
      occurred in vitro as documented by fluorescence in situ hybridization (FISH). The 
      only clonal abnormality occurring in a referent was -Y/-Y,+15 in an elderly male. 
      In conclusion, a constitutional chromosomal instability may precede chromosome 
      abnormalities and be of importance in the development of hairy cell leukemia.
FAU - Haglund, U
AU  - Haglund U
AD  - Department of Hematology, Huddinge Hospital, Sweden.
FAU - Stellan, B
AU  - Stellan B
FAU - Juliusson, G
AU  - Juliusson G
FAU - Gahrton, G
AU  - Gahrton G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow Cells/ultrastructure
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - Leukemia, Hairy Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Trisomy
EDAT- 1998/02/03 00:00
MHDA- 1998/02/03 00:01
CRDT- 1998/02/03 00:00
PHST- 1998/02/03 00:00 [pubmed]
PHST- 1998/02/03 00:01 [medline]
PHST- 1998/02/03 00:00 [entrez]
AID - 10.1038/sj.leu.2400879 [doi]
PST - ppublish
SO  - Leukemia. 1997 Dec;11(12):2105-10. doi: 10.1038/sj.leu.2400879.