PMID- 9450673
OWN - NLM
STAT- MEDLINE
DCOM- 19980310
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 52
IP  - 1
DP  - 1997 Dec 1
TI  - Expression of the glial glutamate transporter EAAT2 in the human CNS: an 
      immunohistochemical study.
PG  - 17-31
AB  - Glutamate transporters play an essential role in terminating the excitatory 
      glutamatergic signal at post-synaptic receptors and in protecting neurones from 
      excitotoxic effects, as well as replenishing the neurotransmitter supply at 
      glutamatergic synapses. The distribution and density of glutamate transporters 
      may be important determinants of vulnerability to glutamate-mediated injury. 
      There is emerging evidence that glutamate transporter dysfunction may be present 
      in motor neurone disease (MND). In this study, a monoclonal antibody, suitable 
      for immunohistochemistry (IHC) in human post-mortem tissue, was produced to the 
      human astrocytic glutamate transporter EAAT2 (excitatory amino acid transporter 
      2). Western blotting of homogenates of human cortical tissue with the EAAT2 
      antibody produced a discrete band at 66 kDa. Detailed IHC analysis of the 
      expression of the EAAT2 protein in the human CNS was undertaken. EAAT2 was 
      exclusively localised to astrocytes, with preferential expression in the caudate 
      nucleus, nucleus basalis of Meynert, spinal ventral horn, cerebral cortex and 
      hippocampus, but with lower levels of expression throughout many other CNS 
      regions. Motor neurone groups vulnerable to neurodegeneration in MND appeared 
      distinctive in being surrounded by extensive, coarse, strongly immunoreactive 
      perisomatic glial profiles. Motor neurone groups which tend to be spared in MND, 
      such as those present in the oculomotor nucleus, showed a lower expression of 
      EAAT2, with fewer perisomatic profiles. The EAAT2 antibody will provide a useful 
      tool for increasing our understanding of the role of EAAT2 in excitatory 
      neurotransmission in health and disease states.
FAU - Milton, I D
AU  - Milton ID
AD  - Novocastra Laboratories, Newcastle upon Tyne, UK.
FAU - Banner, S J
AU  - Banner SJ
FAU - Ince, P G
AU  - Ince PG
FAU - Piggott, N H
AU  - Piggott NH
FAU - Fray, A E
AU  - Fray AE
FAU - Thatcher, N
AU  - Thatcher N
FAU - Horne, C H
AU  - Horne CH
FAU - Shaw, P J
AU  - Shaw PJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Antibodies, Monoclonal)
SB  - IM
MH  - ATP-Binding Cassette Transporters/*analysis
MH  - Amino Acid Transport System X-AG
MH  - Antibodies, Monoclonal
MH  - Basal Ganglia/chemistry
MH  - Biological Transport/physiology
MH  - Blotting, Western
MH  - Central Nervous System/cytology/*metabolism
MH  - Cerebral Cortex/chemistry
MH  - Hippocampus/chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Lumbosacral Region
MH  - Microscopy, Confocal
MH  - Motor Cortex/chemistry
MH  - Neuroglia/*metabolism
MH  - Spinal Cord/chemistry
EDAT- 1998/02/05 00:00
MHDA- 1998/02/05 00:01
CRDT- 1998/02/05 00:00
PHST- 1998/02/05 00:00 [pubmed]
PHST- 1998/02/05 00:01 [medline]
PHST- 1998/02/05 00:00 [entrez]
AID - S0169328X97002337 [pii]
AID - 10.1016/s0169-328x(97)00233-7 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1997 Dec 1;52(1):17-31. doi: 
      10.1016/s0169-328x(97)00233-7.