PMID- 9451456
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 58
IP  - 1
DP  - 1997 Oct 31
TI  - Evaluation of the presence of porcine reproductive and respiratory syndrome virus 
      in packaged pig meat using virus isolation and polymerase chain reaction (PCR) 
      method.
PG  - 1-8
AB  - An investigation was carried out to assess the potential presence of porcine 
      reproductive and respiratory syndrome virus (PRRSV) in packaged pig meat. Samples 
      of meat were collected at the processing plants and were sent to the laboratory 
      for testing by virus isolation and reverse transcription-polymerase chain 
      reaction (RT-PCR). Samples collected at four plants were randomly selected from 
      lots of packaged pig meat from different slaughtering days and were sent frozen 
      to the laboratory. Homogenates of meat were prepared and were inoculated onto 
      MARC-145 cells and after two passages the presence of PRRSV was monitored by 
      indirect immunofluorescence staining using PRRSV specific monoclonal antibody. 
      All pig meat samples (six pools of meat samples from 73 different lots = 438 
      total homogenates) tested were found negative by virus isolation. Primers from 
      open reading frames 6 and 7 were designed and a RT-PCR assay was developed and 
      was demonstrated to detect both North American and European PRRSV isolates. Using 
      this assay virus was detected at a concentration as low as 0.355 infectious 
      virions per ml in supernatant of PRRSV infected cells. This RT-PCR assay could 
      detect PRRS viral nucleic acid from various tissue samples of experimentally 
      infected pigs including muscle tissue, thus demonstrating its applicability on 
      tissue samples. All meat sample homogenates tested by RT-PCR (one sample pool 
      from the 73 lots) were also found negative for PRRS viral nucleic acid. The 
      results suggest that pig meat does not retain detectable amounts of PRRSV and 
      further support that the transmission of PRRSV through pig meat is unlikely.
FAU - Larochelle, R
AU  - Larochelle R
AD  - Health of Animals and Food Laboratory, Agriculture and Agri-Food Canada, 
      Saint-Hyacinthe, Quebec, Canada.
FAU - Magar, R
AU  - Magar R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Abattoirs
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, Viral/analysis
MH  - Cytopathogenic Effect, Viral
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Food Handling
MH  - *Food Microbiology
MH  - Meat/*virology
MH  - Microscopy, Fluorescence/veterinary
MH  - Polymerase Chain Reaction/*veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - RNA, Viral/*analysis
MH  - Swine
MH  - Transcription, Genetic
EDAT- 1998/02/06 00:00
MHDA- 1998/02/06 00:01
CRDT- 1998/02/06 00:00
PHST- 1998/02/06 00:00 [pubmed]
PHST- 1998/02/06 00:01 [medline]
PHST- 1998/02/06 00:00 [entrez]
AID - S0378113597001508 [pii]
AID - 10.1016/s0378-1135(97)00150-8 [doi]
PST - ppublish
SO  - Vet Microbiol. 1997 Oct 31;58(1):1-8. doi: 10.1016/s0378-1135(97)00150-8.