PMID- 9454838
OWN - NLM
STAT- MEDLINE
DCOM- 19980310
LR  - 20220309
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 18
IP  - 4
DP  - 1998 Feb 15
TI  - Neuronal expression of zinc finger transcription factor REST/NRSF/XBR gene.
PG  - 1280-96
AB  - The identification of a common cis-acting silencer element, a neuron-restrictive 
      silencer element (NRSE), in multiple neuron-specific genes, together with the 
      finding that zinc finger transcription factor REST/NRSF/XBR could confer 
      NRSE-mediated silencing in non-neuronal cells, suggested that REST/NRSF/XBR is a 
      master negative regulator of neurogenesis. Here we show that, although 
      REST/NRSF/XBR expression decreases during neuronal development, it proceeds in 
      the adult nervous system. In situ hybridization analysis revealed neuronal 
      expression of rat REST/NRSF/XBR mRNA in adult brain, with the highest levels in 
      the neurons of hippocampus, pons/medulla, and midbrain. The glutamate analog 
      kainic acid increased REST/NRSF/XBR mRNA levels in various hippocampal and 
      cortical neurons in vivo, suggesting that REST/NRSF/XBR has a role in neuronal 
      activity-implied processes. Several alternatively spliced REST/NRSF/XBR mRNAs 
      encoding proteins with nine, five, or four zinc finger motifs are transcribed 
      from REST/NRSF/XBR gene. Two of these transcripts are generated by 
      neuron-specific splicing of a 28-bp-long exon. Rat REST/NRSF/XBR protein isoforms 
      differ in their DNA binding specificities; however, all mediate repression in 
      transient expression assays. Our data suggest that REST/NRSF/XBR is a negative 
      regulator rather than a transcriptional silencer of neuronal gene expression and 
      counteracts with positive regulators to modulate target gene expression 
      quantitatively in different cell types, including neurons.
FAU - Palm, K
AU  - Palm K
AD  - Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and 
      Biophysics, Karolinska Institute, S-171 77 Stockholm, Sweden.
FAU - Belluardo, N
AU  - Belluardo N
FAU - Metsis, M
AU  - Metsis M
FAU - Timmusk, T
AU  - Timmusk T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Xbr-1 protein, Xenopus)
RN  - 0 (Xenopus Proteins)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/cytology/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Eye Proteins/*genetics
MH  - *Gene Expression
MH  - Gene Expression Regulation/physiology
MH  - Homeodomain Proteins/*genetics
MH  - Kainic Acid
MH  - Male
MH  - Molecular Sequence Data
MH  - Neurons/*physiology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Repressor Proteins/*genetics
MH  - Seizures/chemically induced/genetics
MH  - Transcription Factors/*genetics
MH  - *Xenopus Proteins
MH  - Zinc Fingers/*genetics
PMC - PMC6792720
EDAT- 1998/03/14 00:00
MHDA- 1998/03/14 00:01
PMCR- 1998/08/15
CRDT- 1998/03/14 00:00
PHST- 1998/03/14 00:00 [pubmed]
PHST- 1998/03/14 00:01 [medline]
PHST- 1998/03/14 00:00 [entrez]
PHST- 1998/08/15 00:00 [pmc-release]
AID - 1786 [pii]
AID - 10.1523/JNEUROSCI.18-04-01280.1998 [doi]
PST - ppublish
SO  - J Neurosci. 1998 Feb 15;18(4):1280-96. doi: 10.1523/JNEUROSCI.18-04-01280.1998.