PMID- 9457084
OWN - NLM
STAT- MEDLINE
DCOM- 19980223
LR  - 20061115
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 238
IP  - 1
DP  - 1998 Jan 10
TI  - Contribution of growing RNA molecules to the nuclear transcripts foci observed by 
      FISH.
PG  - 299-304
AB  - The focal distributions of RNAs observed using fluorescence in situ hybridization 
      (FISH) in the cell nucleus may correspond either to RNAs in the course of 
      transcription or essentially to accumulation of full-length transcripts at the 
      sites of transcription. To determine to what extent nuclear transcript foci 
      represent growing RNA molecules, uninterrupted hsp70 transcripts were detected by 
      FISH in heat-shocked human fibroblasts using two probes specific for the 5' or 
      for the 3' end of the transcripts. By comparing the size of the signals obtained 
      with each probe, we show that transcript foci mainly represent accumulations of 
      full-length transcripts at their site of transcription. A major contribution of 
      RNAs in the course of transcription to the transcript foci is observed only 
      during the first minutes of gene induction. These observations suggest the 
      existence of a rate-limiting step in the release of newly synthesized transcripts 
      from their site of transcription, which is independent from the step of intron 
      excision.
FAU - Jolly, C
AU  - Jolly C
AD  - DyOGen, INSERM U309, Institut Albert Bonniot, La Tronche, France. 
      caroline.jolly@ujf-grenoble.fr
FAU - Robert-Nicoud, M
AU  - Robert-Nicoud M
FAU - Vourc'h, C
AU  - Vourc'h C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (DNA Primers)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Cell Nucleus/*metabolism/ultrastructure
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Fibroblasts/cytology/metabolism
MH  - HSP70 Heat-Shock Proteins/biosynthesis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Polymerase Chain Reaction
MH  - RNA/*biosynthesis
MH  - Skin/*cytology
MH  - *Transcription, Genetic
EDAT- 1998/02/11 00:00
MHDA- 1998/02/11 00:01
CRDT- 1998/02/11 00:00
PHST- 1998/02/11 00:00 [pubmed]
PHST- 1998/02/11 00:01 [medline]
PHST- 1998/02/11 00:00 [entrez]
AID - S0014-4827(97)93838-6 [pii]
AID - 10.1006/excr.1997.3838 [doi]
PST - ppublish
SO  - Exp Cell Res. 1998 Jan 10;238(1):299-304. doi: 10.1006/excr.1997.3838.