PMID- 9458387
OWN - NLM
STAT- MEDLINE
DCOM- 19980306
LR  - 20191102
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 6
IP  - 5
DP  - 1997 Oct
TI  - Value of fluorescence in situ hybridization for detecting the bcr/abl gene fusion 
      in interphase cells of routine bone marrow specimens.
PG  - 282-7
AB  - Fluorescence in situ hybridization (FISH) is a new technique that allows 
      demonstrating of the bcr/abl gene fusion in bone marrow cells of patients with 
      Philadelphia translocation (Ph)-positive chronic myeloid leukemia (CML). In this 
      study, bone marrow samples of 150 patients were investigated routinely by 
      interphase FISH, cytogenetics, and bone marrow histopathology. In 20 patients 
      with reactive hyperplasia of the granulopoiesis and normal karyotypes, FISH 
      revealed nonspecific bcr/abl fusion signals at a mean frequency of 2.7% of the 
      cells examined. The cutoff level for specific fusion signals was set at three 
      times the standard deviation (9.0%). None of the 29 cytogenetically Ph-negative 
      patients with myeloproliferative disease other than CML had fusion signals 
      exceeding 9%. The mean frequency of specific fusion signals in nontreated 
      patients with CML (n = 59) was 92.7%, and 49.3% in patients with CML who received 
      therapy (n = 42). For diagnosing Ph-positive CML, interphase FISH has been 
      faster, more reliable, and more sensitive than cytogenetics, which was successful 
      in 54 of 59 patients investigated at first diagnosis but only in 27 of 42 
      patients receiving therapy, and it failed to detect Ph-positive cells in three 
      patients with CML. However, small percentages of less than 9.0% of cells with 
      bcr/abl fusion signals were below the threshold of interphase FISH, thereby 
      limiting its use for detecting minimal residual disease.
FAU - Werner, M
AU  - Werner M
AD  - Institute of Pathology, Technische Universitat Munchen, Germany.
FAU - Ewig, M
AU  - Ewig M
FAU - Nasarek, A
AU  - Nasarek A
FAU - Wilkens, L
AU  - Wilkens L
FAU - von Wasielewski, R
AU  - von Wasielewski R
FAU - Tchinda, J
AU  - Tchinda J
FAU - Nolte, M
AU  - Nolte M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
SB  - IM
MH  - Aged
MH  - Bone Marrow Examination/*methods
MH  - Cells, Cultured
MH  - Genes, abl/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics/pathology
MH  - Leukocytes/cytology
MH  - Middle Aged
MH  - Sensitivity and Specificity
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - 10.1097/00019606-199710000-00006 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 1997 Oct;6(5):282-7. doi: 10.1097/00019606-199710000-00006.