PMID- 9458935
OWN - NLM
STAT- MEDLINE
DCOM- 19980227
LR  - 20190921
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 39
IP  - 1
DP  - 1998 Jan
TI  - Incidence of rat-soluble placental factors on IgE and IgG2a synthesis.
PG  - 58-63
AB  - PROBLEM: The in vivo effect of soluble factors present in placental culture 
      supernatants (PCSs) on the synthesis of rat immunoglobulin E (IgE) and IgG2a 
      isotypes was investigated. METHOD OF STUDY: Batches of Wistar SPF rats immunized 
      with a 10-microgram dose of ovalbumin and Al(OH)3 were used: group I, consisted 
      of virgin rats; group II, virgin females injected simultaneously with PCSs; and 
      group III, pregnant females. As controls, nonimmunized batches were included. 
      Serum samples were collected at days 0 (basal) and 10 after antigen challenge, 
      determining levels of total and specific antiovalbumin of both IgE and IgG2a by 
      enzyme-linked immunoadsorbent assay (ELISA). RESULTS: In vivo and at least at the 
      doses administered, PCSs exert an inhibitory effect on the synthesis of specific 
      and total anti-ovalbumin IgE during the course of immune response to such 
      challenge. However, PCSs did not modify serum values of total and specific IgG2a. 
      CONCLUSIONS: These results suggest that PCSs exert selective influence on the 
      synthesis of diverse immunoglobulin isotypes during immune response, through the 
      balance of cytokines synthesized by placental cells.
FAU - Gentile, T
AU  - Gentile T
AD  - Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Departamento de 
      Microbiologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, 
      Argentina.
FAU - Dokmetjian, J
AU  - Dokmetjian J
FAU - Garrido, D
AU  - Garrido D
FAU - Margni, R A
AU  - Margni RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Placental Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antibodies/blood
MH  - Culture Techniques
MH  - Female
MH  - Immunoglobulin E/*biosynthesis/blood
MH  - Immunoglobulin G/*biosynthesis/blood
MH  - Immunoglobulin Isotypes/*biosynthesis/blood
MH  - Ovalbumin/immunology
MH  - Placenta/*drug effects/*metabolism
MH  - Placental Extracts/*pharmacology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Solubility
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - 10.1111/j.1600-0897.1998.tb00334.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1998 Jan;39(1):58-63. doi: 
      10.1111/j.1600-0897.1998.tb00334.x.