PMID- 9459179
OWN - NLM
STAT- MEDLINE
DCOM- 19980227
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 121
IP  - 1
DP  - 1997 Dec 16
TI  - Transient block of respiratory chain by cyanide triggers NADPH-diaphorase 
      activity (a marker for nitric oxide synthase) in Dunning rat prostatic 
      epithelium.
PG  - 91-7
AB  - Synthesis of nitric oxide (NO) has been shown in the glandular epithelium of 
      human prostate, with highest levels in the peripheral zone. This location is 
      believed to be the main source of prostatic cancer. The ability of stromal cells 
      to produce NO may contribute to the malignant process. Since solid tumours are 
      prone to hypoxia and malignant progression, experiments were undertaken to test 
      the effect of respiratory block on the induction of nitric oxide synthase (NOS) 
      by a Dunning rat prostatic epithelial line. A metastatic phenotype (Mat-LyLu) was 
      treated in vitro with brief exposure to cyanide in order to mimic transient 
      hypoxic stress. NADPH-diaphorase activities in paraformaldehyde-fixed cells was 
      used to follow the expression of NOS. NADPH-diaphorase activity was found to be 
      inducible by a range of factors, including mechanical damage and infection of 
      cultures. Cyanide induced a dose-dependent staining that was statistically 
      greater than in untreated cells. Consistent with diaphorase staining being a 
      marker for the inducible isoform of NOS (iNOS), induction and enhancement of 
      staining, respectively, was observed in response to treatment with 
      lipopolysaccharide or withdrawal of dexamethasone supplement. Results demonstrate 
      that prostatic epithelia can be triggered in culture to express iNOS by transient 
      oxidative stress in the form of respiratory poisoning by NaCN. Paradoxically, 
      nitric oxide production by epithelia within hypoxic zones of solid tumours may 
      contribute to the promotion and/or inhibition of tumorigenesis.
FAU - Downing, J E
AU  - Downing JE
AD  - Department of Biology, Imperial College, London, UK. j.downing@ic.ac.uk
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Cyanides)
RN  - 0 (Lipopolysaccharides)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia/*physiology
MH  - Cyanides/pharmacology
MH  - Dexamethasone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Electron Transport/drug effects/*physiology
MH  - Histocytochemistry
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - NADPH Dehydrogenase/*metabolism
MH  - Nitric Oxide Synthase/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Prostatic Neoplasms/enzymology/*pathology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - S0304-3835(97)00339-X [pii]
AID - 10.1016/s0304-3835(97)00339-x [doi]
PST - ppublish
SO  - Cancer Lett. 1997 Dec 16;121(1):91-7. doi: 10.1016/s0304-3835(97)00339-x.