PMID- 9459591
OWN - NLM
STAT- MEDLINE
DCOM- 19980218
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1368
IP  - 1
DP  - 1998 Jan 5
TI  - Quantitative determinations of the steady state transcript levels of hexokinase 
      isozymes and glucose transporter isoforms in normal rat tissues and the malignant 
      tumor cell line AH130.
PG  - 129-36
AB  - The steady state transcript levels of the four hexokinase (HK) isozymes and four 
      glucose transporter (GLUT) isoforms were determined quantitatively by Northern 
      analysis of RNA samples from rat tissues using synthetic fragments of the RNAs 
      encoding the HK isozymes and GLUT isoforms. Results showed that the levels of HK 
      isozyme transcripts were low in rat tissues, the level of that most highly 
      expressed, the type I isozyme (HKI), in the brain being 0.025% of the total 
      poly(A)+ RNA. A good correlation was found between the reported HK activities and 
      the total amounts of transcripts encoding all HK isozymes in various tissues, 
      showing that the HK activities in tissues can be estimated from the total amount 
      of transcripts encoding HK isozymes. The proposed associated expressions of HK 
      isozymes and GLUT isoforms in particular tissues were confirmed at their 
      transcript levels. The steady state transcript levels of type II HK and the type 
      1 GLUT isoform in the malignant tumor cell line AH130 were also determined 
      quantitatively.
FAU - Shinohara, Y
AU  - Shinohara Y
AD  - Faculty of Pharmaceutical Sciences, University of Tokushima, Japan. 
      yasuo@ph.tokushima-u.ac.jp
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Inoo, K
AU  - Inoo K
FAU - Yamazaki, N
AU  - Yamazaki N
FAU - Terada, H
AU  - Terada H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Isoenzymes)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Agar Gel
MH  - Hexokinase/*genetics
MH  - Isoenzymes/*genetics
MH  - Monosaccharide Transport Proteins/*metabolism
MH  - RNA, Messenger/genetics/isolation & purification/*metabolism
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - S0005-2736(97)00189-2 [pii]
AID - 10.1016/s0005-2736(97)00189-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1998 Jan 5;1368(1):129-36. doi: 
      10.1016/s0005-2736(97)00189-2.