PMID- 9460192
OWN - NLM
STAT- MEDLINE
DCOM- 19980323
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 59
IP  - 1
DP  - 1997 Dec
TI  - Baculovirus expression and immunological detection of the major structural 
      proteins of porcine reproductive and respiratory syndrome virus.
PG  - 1-13
AB  - Each of the three major structural proteins (envelope glycoprotein E, 
      nonglycosylated membrane protein M, and nucleoprotein N) of an American strain of 
      porcine reproductive and respiratory syndrome virus (PRRSV) was expressed using a 
      recombinant baculovirus expression system. Insect cells infected with the 
      respective recombinant baculovirus synthesized five distinct forms of 
      glycoprotein E with a molecular mass (M(r)) of either 17, 20, 23, 25 or 26 K, and 
      a single form of nonglycosylated protein M and nucleocapsid N with a M(r) of 
      approximately 21 and 15 K, respectively. Because the number of forms of the 
      glycoprotein E was reduced from five to two (20 and 17 K) when infected cells 
      were treated with tunicamycin, we speculate that the 23, 25 and 26 K forms 
      represent different degrees of glycosylation of the same protein, and that the 20 
      and 17 K peptides represent nonglycosylated forms with and without, respectively, 
      the N-terminal signal sequence. All the proteins were identified by immunoblot 
      with convalescent sera from animals infected with an American strain of PRRSV, 
      indicating that they were similar to the native proteins. The recombinant 
      proteins were purified and used to induce monospecific antisera in rabbits. The 
      ability to produce each protein in the baculovirus system provides an additional 
      means for their structural and functional characterization.
FAU - Kreutz, L C
AU  - Kreutz LC
AD  - Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50010, USA. Lckreutz@creta.ccr.ufsm.br
FAU - Mengeling, W L
AU  - Mengeling WL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies)
RN  - 0 (DNA Primers)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Baculoviridae
MH  - Blotting, Western
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fluorescent Antibody Technique, Indirect
MH  - Molecular Weight
MH  - Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - Rabbits
MH  - Recombinant Proteins/analysis/biosynthesis
MH  - Spodoptera
MH  - Swine
MH  - Transfection
MH  - Viral Structural Proteins/analysis/*biosynthesis
EDAT- 1998/02/14 00:00
MHDA- 1998/02/14 00:01
CRDT- 1998/02/14 00:00
PHST- 1998/02/14 00:00 [pubmed]
PHST- 1998/02/14 00:01 [medline]
PHST- 1998/02/14 00:00 [entrez]
AID - S0378113597001429 [pii]
AID - 10.1016/s0378-1135(97)00142-9 [doi]
PST - ppublish
SO  - Vet Microbiol. 1997 Dec;59(1):1-13. doi: 10.1016/s0378-1135(97)00142-9.