PMID- 9460493
OWN - NLM
STAT- MEDLINE
DCOM- 19980304
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 101
IP  - 1
DP  - 1998 Feb
TI  - A novel three-color, clone-specific fluorescence in situ hybridization procedure 
      for monoclonal gammopathies.
PG  - 7-11
AB  - We have developed a three-color cytoplasmic immunoglobulin (cIg) and fluorescence 
      in situ hybridization (FISH) technique to detect plasma cell chromosomal 
      aneuploidy in patients with multiple myeloma (MM), monoclonal gammopathy of 
      undetermined significance (MGUS), smoldering multiple myeloma (SMM), and 
      amyloidosis (AL). Immunofluorescent-labeled antibodies to detect light chain 
      expression and six directly labeled alpha-satellite chromosome specific 
      enumeration probes (CEP) were used simultaneously to detect aneuploidy of the 
      plasma cells. The six probes were specific for chromosomes 7, 9, 11, 15, 18, and 
      X. The technique was tested in 12 consecutive patient samples (5 MM, 2 MGUS, 3 
      SMM, and 2 AL). Based on the alpha-satellite signals, we found trisomic clones 
      for CEP 7 (4 of 12), CEP 11 (4 of 12), CEP X (1 of 12), CEP 9 (8 of 12), CEP 15 
      (7 of 12), and CEP 18 (5 of 12). Trisomic clones of at least one of the six 
      chromosomes were present in 9 of 12 patients. We believe that this technique 
      efficiently identifies monotypic plasma cells and permits simultaneous analysis 
      of numeric chromosome anomalies by FISH in emerging neoplastic cells. We are in 
      the process of applying this technique to a series of about 100 newly diagnosed 
      monoclonal gammopathy patients.
FAU - Ahmann, G J
AU  - Ahmann GJ
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 
      Minnesota, USA.
FAU - Jalal, S M
AU  - Jalal SM
FAU - Juneau, A L
AU  - Juneau AL
FAU - Christensen, E R
AU  - Christensen ER
FAU - Hanson, C A
AU  - Hanson CA
FAU - Dewald, G W
AU  - Dewald GW
FAU - Greipp, P R
AU  - Greipp PR
LA  - eng
GR  - CA62242/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amyloidosis/*diagnosis
MH  - Aneuploidy
MH  - Bone Marrow Cells/pathology
MH  - Clone Cells
MH  - Female
MH  - Humans
MH  - Immunoglobulin G/*metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*diagnosis
MH  - Paraproteinemias/*diagnosis
MH  - Trisomy/diagnosis
EDAT- 1998/02/14 00:00
MHDA- 1998/02/14 00:01
CRDT- 1998/02/14 00:00
PHST- 1998/02/14 00:00 [pubmed]
PHST- 1998/02/14 00:01 [medline]
PHST- 1998/02/14 00:00 [entrez]
AID - S0165460897000587 [pii]
AID - 10.1016/s0165-4608(97)00058-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Feb;101(1):7-11. doi: 10.1016/s0165-4608(97)00058-7.