PMID- 9460494
OWN - NLM
STAT- MEDLINE
DCOM- 19980304
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 101
IP  - 1
DP  - 1998 Feb
TI  - Fluorescence in situ hybridization on methylcellulose cultured hematopoietic stem 
      cells from myelodysplastic syndromes.
PG  - 12-5
AB  - Myelodysplastic syndromes (MDS) are clonal malignancies characterized by 
      peripheral blood pancytopenia and signs of maturation disturbances of one or 
      several cell lineages in bone marrow. MDS present as chimeras associating normal 
      polyclonal and malignant monoclonal progenitors cells in various proportions. 
      Numerous cytogenetic abnormalities have been reported in MDS and can be detected 
      by fluorescence in situ hybridization (FISH) on interphase cells. We have used 
      this technique on methylcellulose cultured hematopoietic progenitors obtained 
      from three patients suffering from MDS and exhibiting informative karyotypic 
      features. Hematopoietic cells were cultured for 14 days, and individual clones 
      (BFU-E, CFU-GM) were picked up and then cytocentrifuged for FISH analysis. We 
      used centromeric probes realized and labeled in our laboratory by PCR to detect 
      aneuploidies for chromosomes 7 and 11 in two patients. Furthermore, we could 
      detect a 5q partial deletion on interphase cells from the third patient using a 
      5q31 specific probe visualized with the HNPP Fluorescent Detection Set from 
      Boehringer Mannheim. In conclusion, FISH is a helpful method to detect malignant 
      clones in hematopoietic progenitor cultures and hence to study the relative 
      growth of normal vs. leukemic cells in MDS.
FAU - Dupont, J M
AU  - Dupont JM
AD  - Service d'Histologie Embryologie Cytogenetique, Paris, France.
FAU - Fontenay-Roupie, M
AU  - Fontenay-Roupie M
FAU - Dupuy, O
AU  - Dupuy O
FAU - Lebarr, A
AU  - Lebarr A
FAU - Le Tessier, D
AU  - Le Tessier D
FAU - Auvinet, P
AU  - Auvinet P
FAU - Rabineau, D
AU  - Rabineau D
FAU - Fichelson, S
AU  - Fichelson S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 9004-67-5 (Methylcellulose)
SB  - IM
MH  - Aged
MH  - Aneuploidy
MH  - Cells, Cultured
MH  - Centromere
MH  - Chromosome Aberrations/diagnosis
MH  - Chromosome Disorders
MH  - Female
MH  - Hematopoietic Stem Cells/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Methylcellulose
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*diagnosis
EDAT- 1998/02/14 00:00
MHDA- 1998/02/14 00:01
CRDT- 1998/02/14 00:00
PHST- 1998/02/14 00:00 [pubmed]
PHST- 1998/02/14 00:01 [medline]
PHST- 1998/02/14 00:00 [entrez]
AID - S0165460897000617 [pii]
AID - 10.1016/s0165-4608(97)00061-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Feb;101(1):12-5. doi: 10.1016/s0165-4608(97)00061-7.