PMID- 9462212
OWN - NLM
STAT- MEDLINE
DCOM- 19980219
LR  - 20190501
IS  - 0017-5749 (Print)
IS  - 1458-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 41
IP  - 6
DP  - 1997 Dec
TI  - Cytokine mRNA expression in intestinal tissue of interleukin-2 deficient mice 
      with bowel inflammation.
PG  - 793-800
AB  - BACKGROUND: Mice deficient in interleukin-2 (IL-2) develop inflammatory bowel 
      disease resembling ulcerative colitis in humans. Recent studies provided evidence 
      that alpha beta T cells, particularly CD4 T cells, rather than B cells, are 
      involved in the pathogenesis of bowel inflammation of IL-2 deficient mice. AIM: 
      To analyse the pattern of expression of cytokine mRNA in intestinal tissue of 
      normal and IL-2 deficient mice. METHODS: Expression of beta-actin, IL-1 alpha, 
      IL-1 beta, IL-6, IL-10, tumour necrosis factor alpha (TNF-alpha), interferon 
      gamma (IFN-gamma) and transforming growth factor beta 1 (TGF-beta 1) mRNA was 
      analysed in colon and small intestinal tissue of both IL-2 deficient (IL-2-/-) 
      mice and normal (wild type) litter mates (IL-2+/+) at different ages by using 
      qualitative, as well as semiquantitative, competitive reverse transcription 
      polymerase chain reaction (RT-PCR). Results were correlated with the phase of 
      progression of the disease, as determined by histology. RESULTS: IL-2-/- mice had 
      expressed low levels of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, and IFN-gamma 
      mRNA in the colon by 1.5 weeks of age. In advance of the development of 
      histologically and clinically detectable bowel inflammation, expression of IL-1 
      alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10, but not TGF-beta 1, mRNA 
      increased in the colon of IL-2 deficient mice. In contrast, IL-2+/+ mice 
      expressed TGF-beta 1 mRNA in colon tissue at 13 and 23 weeks of age, but not IL-1 
      alpha, IL-1 beta, IL-6, TNF-alpha, IL-10, or IFN-gamma mRNA. Levels of expression 
      of cytokine mRNA in tissue from the small intestine were comparable in IL-2-/- 
      and IL-2+/+ mice. CONCLUSIONS: Bowel inflammation in IL-2 deficient mice is 
      preceded by an increase in IL-1 alpha, IL-1 beta, TNF-alpha, and IFN-gamma mRNA 
      expression in colon tissue. Low levels of TGF-beta 1, but high levels of IL-1 
      alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10 mRNA expression correlate 
      with the manifestation of severe colitis, and suggest that T cells and 
      macrophages are involved in bowel inflammation of IL-2 deficient mice.
FAU - Autenrieth, I B
AU  - Autenrieth IB
AD  - Max v. Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig 
      Maximilians Universitat, Munchen, Germany.
FAU - Bucheler, N
AU  - Bucheler N
FAU - Bohn, E
AU  - Bohn E
FAU - Heinze, G
AU  - Heinze G
FAU - Horak, I
AU  - Horak I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (Actins)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Colon/immunology
MH  - Cytokines/genetics/*metabolism
MH  - Gene Expression
MH  - Inflammatory Bowel Diseases/*immunology/metabolism
MH  - Interferon-gamma/genetics
MH  - Interleukin-1/genetics
MH  - Interleukin-10/genetics
MH  - Interleukin-2/*deficiency
MH  - Interleukin-6/genetics
MH  - Intestinal Mucosa/metabolism
MH  - Intestine, Small/immunology
MH  - Intestines/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis/*metabolism
MH  - Transforming Growth Factor beta/genetics
MH  - Tumor Necrosis Factor-alpha/genetics
PMC - PMC1891612
EDAT- 1998/02/14 00:00
MHDA- 1998/02/14 00:01
PMCR- 2000/12/01
CRDT- 1998/02/14 00:00
PHST- 1998/02/14 00:00 [pubmed]
PHST- 1998/02/14 00:01 [medline]
PHST- 1998/02/14 00:00 [entrez]
PHST- 2000/12/01 00:00 [pmc-release]
AID - 10.1136/gut.41.6.793 [doi]
PST - ppublish
SO  - Gut. 1997 Dec;41(6):793-800. doi: 10.1136/gut.41.6.793.