PMID- 9462545
OWN - NLM
STAT- MEDLINE
DCOM- 19980220
LR  - 20190822
IS  - 0361-8609 (Print)
IS  - 0361-8609 (Linking)
VI  - 57
IP  - 2
DP  - 1998 Feb
TI  - Immunologic effects of anti-D (WinRho-SD) in children with immune 
      thrombocytopenic purpura.
PG  - 131-8
AB  - Intravenous immunoglobulin (IVIG) is an effective treatment for immune 
      thrombocytopenic purpura (ITP) that induces transient blockade of the 
      reticuloendothelial system (RES) with additional effects including alteration of 
      T lymphocyte subsets and suppression of in vitro T lymphocyte proliferation. As 
      anti-D also is an effective treatment for ITP, we investigated its in vitro and 
      in vivo immunologic effects. The in vitro effects of various agents used in ITP 
      therapy were compared using T lymphocyte proliferation assays. Anti-D caused 
      significantly less inhibition than IVIG or dexamethasone, but non-specific 
      protein was as suppressive as IVIG. Six children with chronic ITP were studied 
      following anti-D administration. Patients received a single dose of anti-D 
      (WinRho-SD, 50 microg/kg i.v. over 5 min) and were studied on day 0, day 7, and 1 
      month later. Anti-D did not affect T lymphocyte subsets including the T cell 
      receptor variable beta repertoire, in vitro T lymphocyte proliferation to 
      mitogens, recall antigens, or interleukin-2, in vitro IgG synthesis induced by 
      pokeweed mitogen, or T lymphocyte cytokine mRNA levels. We conclude that anti-D 
      has no demonstrable in vitro or in vivo effects on lymphocyte enumeration or 
      function, and therefore likely is effective in the treatment of ITP primarily 
      through RES blockade.
FAU - Zimmerman, S A
AU  - Zimmerman SA
AD  - Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 
      27710, USA. Zimme008@mc.duke.edu
FAU - Malinoski, F J
AU  - Malinoski FJ
FAU - Ware, R E
AU  - Ware RE
LA  - eng
GR  - 5-T32-CA09307-19/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Hematol
JT  - American journal of hematology
JID - 7610369
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Isoantibodies)
RN  - 0 (RHO(D) antibody)
RN  - 0 (Rho(D) Immune Globulin)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/immunology
MH  - Humans
MH  - Immunoglobulins, Intravenous/*administration & dosage
MH  - Immunophenotyping
MH  - Infant
MH  - Isoantibodies/*administration & dosage/immunology/therapeutic use
MH  - Purpura, Thrombocytopenic, Idiopathic/*drug therapy/*immunology
MH  - Rho(D) Immune Globulin
MH  - T-Lymphocyte Subsets/*immunology
EDAT- 1998/02/14 05:48
MHDA- 2000/06/20 09:00
CRDT- 1998/02/14 05:48
PHST- 1998/02/14 05:48 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/02/14 05:48 [entrez]
AID - 10.1002/(SICI)1096-8652(199802)57:2<131::AID-AJH7>3.0.CO;2-X [pii]
AID - 10.1002/(sici)1096-8652(199802)57:2<131::aid-ajh7>3.0.co;2-x [doi]
PST - ppublish
SO  - Am J Hematol. 1998 Feb;57(2):131-8. doi: 
      10.1002/(sici)1096-8652(199802)57:2<131::aid-ajh7>3.0.co;2-x.