PMID- 9463410
OWN - NLM
STAT- MEDLINE
DCOM- 19980323
LR  - 20240213
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 187
IP  - 4
DP  - 1998 Feb 16
TI  - Abnormalities in monocyte recruitment and cytokine expression in monocyte 
      chemoattractant protein 1-deficient mice.
PG  - 601-8
AB  - Monocyte chemoattractant protein 1 (MCP-1) is a CC chemokine that attracts 
      monocytes, memory T lymphocytes, and natural killer cells. Because other 
      chemokines have similar target cell specificities and because CCR2, a cloned 
      MCP-1 receptor, binds other ligands, it has been uncertain whether MCP-1 plays a 
      unique role in recruiting mononuclear cells in vivo. To address this question, we 
      disrupted SCYA2 (the gene encoding MCP-1) and tested MCP-1-deficient mice in 
      models of inflammation. Despite normal numbers of circulating leukocytes and 
      resident macrophages, MCP-1(-/-) mice were specifically unable to recruit 
      monocytes 72 h after intraperitoneal thioglycollate administration. Similarly, 
      accumulation of F4/80+ monocytes in delayed-type hypersensitivity lesions was 
      impaired, although the swelling response was normal. Development of secondary 
      pulmonary granulomata in response to Schistosoma mansoni eggs was blunted in 
      MCP-1(-/-) mice, as was expression of IL-4, IL-5, and interferon gamma in 
      splenocytes. In contrast, MCP-1(-/-) mice were indistinguishable from wild-type 
      mice in their ability to clear Mycobacterium tuberculosis. Our data indicate that 
      MCP-1 is uniquely essential for monocyte recruitment in several inflammatory 
      models in vivo and influences expression of cytokines related to T helper 
      responses.
FAU - Lu, B
AU  - Lu B
AD  - Perlmutter Laboratory, Children's Hospital, Harvard Medical School, Boston, 
      Massachusetts 02115, USA.
FAU - Rutledge, B J
AU  - Rutledge BJ
FAU - Gu, L
AU  - Gu L
FAU - Fiorillo, J
AU  - Fiorillo J
FAU - Lukacs, N W
AU  - Lukacs NW
FAU - Kunkel, S L
AU  - Kunkel SL
FAU - North, R
AU  - North R
FAU - Gerard, C
AU  - Gerard C
FAU - Rollins, B J
AU  - Rollins BJ
LA  - eng
GR  - HL-51366/HL/NHLBI NIH HHS/United States
GR  - R37 HL035276/HL/NHLBI NIH HHS/United States
GR  - HL-35276/HL/NHLBI NIH HHS/United States
GR  - CA-53091/CA/NCI NIH HHS/United States
GR  - R01 CA053091/CA/NCI NIH HHS/United States
GR  - R01 HL051366/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, Helminth)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-5)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/immunology
MH  - Chemokine CCL2/deficiency/genetics/*physiology
MH  - Cytokines/*biosynthesis
MH  - Dermatitis, Contact/immunology
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Targeting
MH  - Granuloma, Respiratory Tract/immunology/parasitology
MH  - Hypersensitivity, Delayed/immunology
MH  - Inflammation/*immunology
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Interleukin-5/biosynthesis
MH  - Male
MH  - Mice
MH  - Monocytes/immunology/*physiology
MH  - Mycobacterium bovis/immunology
MH  - Phenotype
MH  - Schistosomiasis mansoni/immunology
MH  - Tuberculosis/immunology
PMC - PMC2212142
EDAT- 1998/03/28 00:00
MHDA- 1998/03/28 00:01
PMCR- 1998/08/16
CRDT- 1998/03/28 00:00
PHST- 1998/03/28 00:00 [pubmed]
PHST- 1998/03/28 00:01 [medline]
PHST- 1998/03/28 00:00 [entrez]
PHST- 1998/08/16 00:00 [pmc-release]
AID - 10.1084/jem.187.4.601 [doi]
PST - ppublish
SO  - J Exp Med. 1998 Feb 16;187(4):601-8. doi: 10.1084/jem.187.4.601.