PMID- 9464465
OWN - NLM
STAT- MEDLINE
DCOM- 19980219
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 62
IP  - 6
DP  - 1998
TI  - Mechanism of lanthanum inhibition of extracellular ATP-evoked calcium 
      mobilization in MDCK cells.
PG  - 533-40
AB  - We have studied the effects of La3+ on ATP-evoked rises in intracellular calcium 
      levels ([Ca2+]i) measured by fura-2 fluorimetry in Madin Darby canine kidney 
      (MDCK) cells. ATP evoked [Ca2+]i rises dose-dependently with an EC50 of 2.5 
      microM. The trigger for the Ca2+ signal was a release of Ca2+ from the 
      inositol-1,4,5-trisphosphate (IP3)-sensitive stores because the signal was 
      completely blocked by pretreatment with the endoplasmic reticulum (ER) Ca2+ pump 
      inhibitor thapsigargin (TG) or the phospholipase C (PLC) inhibitor U73122. Both 
      the peak height and area under the curve of 10 microM ATP-evoked Ca2+ signal was 
      reduced by approximately 50% by extracellular Ca2+ removal, suggesting that ATP 
      induced capacitative Ca2+ entry. La3+ inhibited the ATP-evoked Ca2+ signal 
      dose-dependently when added before or after ATP. Pretreatment of 0.1 mM La3+ 
      inhibited approximately 90% of the Ca2+ signal induced by 10 microM ATP. The 
      mechanisms underlying the La3+ inhibition appear to involve not only block of 
      capacitative Ca2+ entry but also interference with ATP binding to the ATP 
      receptors.
FAU - Jan, C R
AU  - Jan CR
AD  - Department of Medical Education and Research, Veterans General 
      Hospital-Kaohsiung, National Sun Yat-Sen University, Taiwan, ROC. 
      crjan@isca.vghks.gov.tw
FAU - Ho, C M
AU  - Ho CM
FAU - Wu, S N
AU  - Wu SN
FAU - Huang, J K
AU  - Huang JK
FAU - Tseng, C J
AU  - Tseng CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 6I3K30563S (Lanthanum)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Kidney/metabolism
MH  - Lanthanum/*pharmacology
EDAT- 1998/02/17 00:00
MHDA- 1998/02/17 00:01
CRDT- 1998/02/17 00:00
PHST- 1998/02/17 00:00 [pubmed]
PHST- 1998/02/17 00:01 [medline]
PHST- 1998/02/17 00:00 [entrez]
AID - S0024320597011491 [pii]
AID - 10.1016/s0024-3205(97)01149-1 [doi]
PST - ppublish
SO  - Life Sci. 1998;62(6):533-40. doi: 10.1016/s0024-3205(97)01149-1.