PMID- 9464490
OWN - NLM
STAT- MEDLINE
DCOM- 19980219
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 122
IP  - 1-2
DP  - 1998 Jan 9
TI  - Integrated and episomal forms of Epstein-Barr virus (EBV) in EBV associated 
      disease.
PG  - 43-50
AB  - Epstein-Barr virus (EBV) is known to be linear in viral particles but EBV 
      circularizes into an episomal form after infection. Recently, the presence of 
      integrated EBV DNA has been reported. We investigated EBV integration into the 
      human genome in EBV-associated disease using Southern blotting. One hundred four 
      cases in which the presence of EBV was confirmed by Southern blotting with EBV-W 
      probes were thus analyzed with left- and right-hand end probes of linear EBV. 
      Integrated EBV was demonstrated in 11 of 104 cases; five of 14 cases with B cell 
      lymphoma (36%), one of 12 cases with nasopharyngeal carcinomas (8%), four of 31 
      cases with natural killer (NK) leukemia/lymphoma (13%) and one of 11 cases with 
      chronic EBV infection (9%). However, none of the 24 T cell lymphoma, seven 
      Hodgkin's disease, or five acute EBV infection cases showed integrated EBV. In 
      addition, seven of the 11 cases with EBV integration (five B cell lymphoma and 
      two NK leukemia/lymphoma) showed only an integration form, however, the other 
      four (two NK leukemia/lymphoma, one nasopharyngeal carcinoma and one chronic EBV 
      infection) showed both integrated and episomal forms. The integrated form was 
      frequently found in B cell lymphoma and especially in high grade B cell lymphoma. 
      Fluorescence in situ hybridization (FISH) was performed in two cases (NK and B 
      cell lymphoma), which represented integrated EBV in Southern blotting and the 
      integration form was confirmed in both. However, it is still uncertain as to 
      whether or not the EBV integration site is directly associated with chromosomal 
      abnormality.
FAU - Ohshima, K
AU  - Ohshima K
AD  - Department of Pathology, School of Medicine, Fukuoka University, Japan.
FAU - Suzumiya, J
AU  - Suzumiya J
FAU - Kanda, M
AU  - Kanda M
FAU - Kato, A
AU  - Kato A
FAU - Kikuchi, M
AU  - Kikuchi M
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
SB  - IM
MH  - Blotting, Southern
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 4, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Plasmids
MH  - Tumor Virus Infections/*virology
MH  - *Virus Integration
EDAT- 1998/02/17 00:00
MHDA- 1998/02/17 00:01
CRDT- 1998/02/17 00:00
PHST- 1998/02/17 00:00 [pubmed]
PHST- 1998/02/17 00:01 [medline]
PHST- 1998/02/17 00:00 [entrez]
AID - S0304-3835(97)00368-6 [pii]
AID - 10.1016/s0304-3835(97)00368-6 [doi]
PST - ppublish
SO  - Cancer Lett. 1998 Jan 9;122(1-2):43-50. doi: 10.1016/s0304-3835(97)00368-6.