PMID- 9465067
OWN - NLM
STAT- MEDLINE
DCOM- 19980319
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 95
IP  - 4
DP  - 1998 Feb 17
TI  - Menin, the product of the MEN1 gene, is a nuclear protein.
PG  - 1630-4
AB  - The MEN1 gene, mutations in which are responsible for multiple endocrine 
      neoplasia type 1 (MEN1), encodes a 610-amino acid protein, denoted menin. The 
      amino acid sequence of this putative tumor suppressor offers no clue to the 
      function or subcellular location of the protein. We report herein, based on 
      immunofluorescence, Western blotting of subcellular fractions, and epitope 
      tagging with enhanced green fluorescent protein, that menin is located primarily 
      in the nucleus. Enhanced green fluorescent protein-tagged menin deletion 
      constructs identify at least two independent nuclear localization signals (NLS), 
      both located in the C-terminal fourth of the protein. Among the 68 known 
      independent disease-associated mutations, none of the 22 missense and 3 in-frame 
      deletions affect either of the putative NLS sequences. However, if expressed, 
      none of the truncated menin proteins resulting from the 43 known 
      frameshift/nonsense mutations would retain both the NLSs. The precise role(s) of 
      menin in the nucleus remain to be understood.
FAU - Guru, S C
AU  - Guru SC
AD  - Genetics and Molecular Biology Branch, National Human Genome Research Institute, 
      National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Goldsmith, P K
AU  - Goldsmith PK
FAU - Burns, A L
AU  - Burns AL
FAU - Marx, S J
AU  - Marx SJ
FAU - Spiegel, A M
AU  - Spiegel AM
FAU - Collins, F S
AU  - Collins FS
FAU - Chandrasekharappa, S C
AU  - Chandrasekharappa SC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Luminescent Proteins)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Compartmentation
MH  - Cell Nucleus/*metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/immunology/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Peptides/chemistry/immunology
MH  - *Proto-Oncogene Proteins
PMC - PMC19125
EDAT- 1998/03/21 00:00
MHDA- 1998/03/21 00:01
PMCR- 1998/08/17
CRDT- 1998/03/21 00:00
PHST- 1998/03/21 00:00 [pubmed]
PHST- 1998/03/21 00:01 [medline]
PHST- 1998/03/21 00:00 [entrez]
PHST- 1998/08/17 00:00 [pmc-release]
AID - 4314 [pii]
AID - 10.1073/pnas.95.4.1630 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1630-4. doi: 10.1073/pnas.95.4.1630.