PMID- 9465891
OWN - NLM
STAT- MEDLINE
DCOM- 19980304
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 78
IP  - 3-4
DP  - 1997
TI  - Chromosomes of Brants' whistling rat and genome conservation in the Otomyinae 
      revealed by G-banding and fluorescence in situ hybridization.
PG  - 216-20
AB  - Conventional G- and C-banding were used to describe the chromosomes of the 
      whistling rat, Parotomys brantsii. This species has a diploid number of 42 
      chromosomes. C-banding showed that large blocks of pericentromeric 
      heterochromatin or entirely heterochromatic short arms characterize most 
      chromosomes. G-banding and fluorescence in situ hybridization (FISH) 
      incorporating two laboratory mouse chromosome paints were used to compare the 
      genomes of P. brantsii, the bush karoo rat (Otomys unisulcatus; 2n = 28), and the 
      vlei rat (O. irroratus; 2n = 28-31). The FISH results showed that sequences 
      corresponding to mouse chromosome 2 are conserved as a single chromosome in O. 
      irroratus but are found on two separate chromosomes in P. brantsii and O. 
      unisulcatus. In contrast, a mouse chromosome 6 paint showed hybridization to 
      single chromosomes in all three species examined. When taken together, the FISH 
      and cytogenetic results produce two important findings: (1) previously undetected 
      homoeologies between the two Otomys species can be identified and (2) a high 
      proportion of conserved chromosomes exists between P. brantsii and O. 
      unisulcatus. This, in conjunction with previously reported allozyme and 
      immunoblot data, raises serious questions about the validity of the current 
      generic taxonomy of the Otomyinae.
FAU - Rambau, R V
AU  - Rambau RV
AD  - Department of Zoology and Entomology, University of Pretoria, South Africa.
FAU - Harrison, W R
AU  - Harrison WR
FAU - Elder, F F
AU  - Elder FF
FAU - Robinson, T J
AU  - Robinson TJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Animals
MH  - Animals, Wild/*genetics
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Rats/*genetics
MH  - Species Specificity
EDAT- 1997/01/01 00:00
MHDA- 1998/02/18 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/02/18 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000134660 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1997;78(3-4):216-20. doi: 10.1159/000134660.