PMID- 9466000
OWN - NLM
STAT- MEDLINE
DCOM- 19980311
LR  - 20190914
IS  - 0148-5016 (Print)
IS  - 0148-5016 (Linking)
VI  - 40
IP  - 1
DP  - 1998 Jan-Feb
TI  - Susceptibility of sperm chromatin to acid denaturation in situ: a study in 
      endogenous FSH-deprived adult male bonnet monkeys (Macaca radiata).
PG  - 29-41
AB  - Acid denaturation of calf thymus DNA in vitro followed by acridine orange (AO) 
      binding induced a 112% increase in the emission of red, a 58% decrease in green, 
      and a consequential decrease in the ratio of green:red fluorescences from 1.7 to 
      0.9. This metachromatic property of AO on binding to DNA following acid 
      denaturation was utilized to study the susceptibility of normal and ovine 
      follicle-stimulating hormone (oFSH) actively immunized bonnet monkey spermatozoa 
      voided throughout the year. For analyses, the scattergram generated by the 
      emission of red and green fluorescences by 10,000 AO-bound sperm from each semen 
      sample was divided into 4 quadrant zones representing percentage cells 
      fluorescing high green-low red (Q1), high green-high red (Q2), low green-low red 
      (Q3) and low green-high red. (Q4). Normal monkey sperm obtained during the months 
      of July-December exhibited 76, 13, and 11% cells in Q2, Q3, and Q4 quadrants, 
      respectively. However, during January-June, when the females of the species are 
      markedly subfertile, noncycling, and amenorrhoeic, the spermatozoa ejaculated by 
      the male monkeys exhibited 38, 39, and 23% sperm in Q2, Q3, and Q4, respectively, 
      the differences being highly significant (p < .01-.001). FSH deprivation induced 
      significant shifts in fluorescence emissions, from respective controls, with 39, 
      33, and 28% cells in Q2, Q3, and Q4, respectively, during July-December, and 15, 
      48, and 37% sperm in Q2, Q3, and Q4 quadrants, respectively, during January-June. 
      It is postulated that the altered kinetics of germ cell transformations and the 
      deficient spermiogenesis observed earlier following FSH deprivation in these 
      monkeys may have induced the enhanced susceptibility to acid denaturation in 
      sperm.
FAU - Aravindan, G R
AU  - Aravindan GR
AD  - Center for Reproductive Biology and Molecular Endocrinology, Indian Institute of 
      Science, Bangalore, India. aravind@wsu.edu
FAU - Moudgal, N R
AU  - Moudgal NR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Arch Androl
JT  - Archives of andrology
JID - 7806755
RN  - 0 (Acids)
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Chromatin)
RN  - 0 (Coloring Agents)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Acids
MH  - Animals
MH  - Antibodies, Blocking/analysis
MH  - Chromatin/*chemistry
MH  - Coloring Agents
MH  - Fertility/physiology
MH  - Flow Cytometry
MH  - Follicle Stimulating Hormone/*deficiency/immunology
MH  - Immunization
MH  - Infertility, Male/physiopathology
MH  - Macaca radiata
MH  - Male
MH  - Nucleic Acid Denaturation/*physiology
MH  - Seasons
MH  - Semen/chemistry
MH  - Spectrometry, Fluorescence
MH  - Spermatozoa/*metabolism/ultrastructure
EDAT- 1998/02/18 00:00
MHDA- 1998/02/18 00:01
CRDT- 1998/02/18 00:00
PHST- 1998/02/18 00:00 [pubmed]
PHST- 1998/02/18 00:01 [medline]
PHST- 1998/02/18 00:00 [entrez]
AID - 10.3109/01485019808987925 [doi]
PST - ppublish
SO  - Arch Androl. 1998 Jan-Feb;40(1):29-41. doi: 10.3109/01485019808987925.