PMID- 9467358
OWN - NLM
STAT- MEDLINE
DCOM- 19980218
LR  - 20191102
IS  - 1354-523X (Print)
IS  - 1354-523X (Linking)
VI  - 3
IP  - 3
DP  - 1997 Sep
TI  - Paraffin-embedded tissue as a source of RNA for gene expression analysis in oral 
      malignancy.
PG  - 157-61
AB  - OBJECTIVE: To assess the feasibility of using archival oral mucosal tissue to 
      examine gene expression at the ribonucleic acid (RNA) level. MATERIALS AND 
      METHODS: We describe the isolation of RNA from 8 microns sections of 
      formalin-fixed paraffin-embedded oral mucosal tissue. RNA was reverse transcribed 
      and three candidate genes amplified by polymerase chain reaction (PCR). The 
      ribosomal protein S14 gene is a housekeeping gene which has been used as an 
      internal standard in several quantitative PCR protocols. The thymidine kinase 
      (TK) gene is expressed at low levels in most tissues and, with a well-documented 
      genomic organisation, is a useful tool for discrimination between genomic DNA and 
      cDNA. The RI alpha gene is reported to be overexpressed in many cancer cell 
      lines, in malignant tissue and in vitro transformed cells. RESULTS: The S14 gene, 
      the TK gene and the RI alpha gene of the cAMP-dependent protein kinase (PKA) were 
      amplified successfully from formalin-fixed paraffin-embedded tissue sections. The 
      TK primer pair is a useful additional tool in the unambiguous identification of 
      RNA-derived species. CONCLUSION: RNA suitable for reverse transcribed (RT)-PCR 
      was extracted from archival oral mucosal tissue. This should permit rapid 
      sequence analysis of transcribed tumor suppressor genes and oncogenes in this 
      material. Furthermore, the RT-PCR approach described may allow quantification of 
      gene expression in oral mucosal archival material processed in a standard 
      fashion.
FAU - Cairns, M T
AU  - Cairns MT
AD  - Department of Oncology, Queen's University of Belfast, Belfast City Hospital 
      Tower, UK.
FAU - Church, S
AU  - Church S
FAU - Johnston, P G
AU  - Johnston PG
FAU - Phenix, K V
AU  - Phenix KV
FAU - Marley, J J
AU  - Marley JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (ribosomal protein S14)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cyclic AMP-Dependent Protein Kinases/genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Middle Aged
MH  - Oncogenes/*genetics
MH  - *Paraffin Embedding
MH  - Pilot Projects
MH  - Polymerase Chain Reaction/methods
MH  - Protein-Tyrosine Kinases/genetics
MH  - RNA, Messenger/analysis/*isolation & purification
MH  - RNA, Neoplasm/analysis/*isolation & purification
MH  - RNA-Directed DNA Polymerase
MH  - Ribosomal Proteins/genetics
MH  - Tongue Neoplasms/*genetics
EDAT- 1998/02/19 00:00
MHDA- 1998/02/19 00:01
CRDT- 1998/02/19 00:00
PHST- 1998/02/19 00:00 [pubmed]
PHST- 1998/02/19 00:01 [medline]
PHST- 1998/02/19 00:00 [entrez]
AID - 10.1111/j.1601-0825.1997.tb00028.x [doi]
PST - ppublish
SO  - Oral Dis. 1997 Sep;3(3):157-61. doi: 10.1111/j.1601-0825.1997.tb00028.x.