PMID- 9467814
OWN - NLM
STAT- MEDLINE
DCOM- 19980312
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 17
IP  - 12
DP  - 1997 Dec
TI  - Characterization of fetal haematopoietic progenitors circulating in maternal 
      blood of seven aneuploid pregnancies.
PG  - 1159-69
AB  - A retrospective study was carried out in order to investigate the phenotype of 
      fetal haematopoietic progenitors circulating in the maternal blood of seven 
      aneuploid pregnancies. Five of the blood samples were taken during pregnancies 
      affected by various fetal aneuploidies, while the other two were collected after 
      therapeutic abortion due to prenatal cytogenetic diagnosis of trisomies 21 and 
      18. Haematopoietic progenitor cells, isolated by labelling the erythropoietin 
      receptors with the biotinylated ligand before magnetic sorting and/or fibronectin 
      cell adhesion assay, were cultured in a suitable semisolid medium. Single- or 
      dual-colour fluorescence in situ hybridization (FISH) was utilized to identify 
      and enumerate fetal cells amplified in culture. Fetal trisomies were confirmed in 
      the FISH analysis with chromosome-specific probes in all the cases analysed. The 
      fetal purity rate ranged from 16 to 26 per cent. Haematopoietic progenitors of 
      fetal origin were found to include CFU-E, CFU-GEMM, and possibly also M-BFU-E. 
      Interestingly, a more immature progenitor with high self-renewal capacity 
      (CFU-blast cell) isolated by fibronectin sorting was shown to have a relatively 
      high frequency in one case of Down syndrome. In general, the results of this 
      study demonstrate the feasibility of diagnosing the major fetal chromosomopathies 
      by culturing fetal cells taken from maternal blood. Furthermore, our initial data 
      on the sequential sorting for fibronectin and erythropoietin receptors lead us to 
      believe that this approach may broaden the range of fetal haematopoietic 
      progenitors retrievable from the maternal circulation.
FAU - Valerio, D
AU  - Valerio D
AD  - Istituto di Ricerche Genetiche, Napoli, Italy.
FAU - Altieri, V
AU  - Altieri V
FAU - Antonucci, F R
AU  - Antonucci FR
FAU - Aiello, R
AU  - Aiello R
LA  - eng
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cells, Cultured
MH  - *Chromosomes, Human, Pair 13
MH  - *Chromosomes, Human, Pair 18
MH  - Down Syndrome/*diagnosis/embryology/genetics
MH  - Female
MH  - Fetal Diseases/*diagnosis/embryology/genetics
MH  - Gestational Age
MH  - Hematopoietic Stem Cells/chemistry/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Phenotype
MH  - Pregnancy/blood
MH  - Prenatal Diagnosis/*methods
MH  - Retrospective Studies
EDAT- 1998/02/19 04:53
MHDA- 2000/06/20 09:00
CRDT- 1998/02/19 04:53
PHST- 1998/02/19 04:53 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/02/19 04:53 [entrez]
AID - 10.1002/(SICI)1097-0223(199712)17:12<1159::AID-PD250>3.0.CO;2-L [pii]
AID - 10.1002/(sici)1097-0223(199712)17:12<1159::aid-pd250>3.0.co;2-l [doi]
PST - ppublish
SO  - Prenat Diagn. 1997 Dec;17(12):1159-69. doi: 
      10.1002/(sici)1097-0223(199712)17:12<1159::aid-pd250>3.0.co;2-l.