PMID- 9467822
OWN - NLM
STAT- MEDLINE
DCOM- 19980312
LR  - 20131121
IS  - 1078-0297 (Print)
IS  - 1078-0297 (Linking)
VI  - 98
IP  - 2
DP  - 1997 Nov
TI  - Involvement of tyrosine kinase in capacitative Ca2+ entry pathway in rat glioma 
      C6 cells.
PG  - 127-40
AB  - Capacitative Ca2+ entry, a main pathway of Ca2+ entry evoked by receptor 
      activation, is widely confirmed in various types of cells. However, the mechanism 
      of the activation of capacitative Ca2+ entry is unknown. We checked the several 
      candidates for the mechanism of capacitative Ca2+ entry pathway in rat glioma C6 
      cells using thapsigargin (TG), a microsomal Ca(2+)-ATPase inhibitor. Pretreatment 
      with pertussis toxin did not affect the peak and sustained elevation of [Ca2+]i 
      evoked by TG. Sodium nitroprusside and 8-bromo cyclic GMP did not affect an 
      elevation of [Ca2+]i induced by TG. Phorbol 12-myristate 13-acetate, an activator 
      of protein kinase C (PKC), and staurosporine, an inhibitor of PKC, did not modify 
      an increase in [Ca2+]i induced by TG. Okadaic acid, an inhibitor of phosphatase, 
      did not affect an increase in [Ca2+]i evoked by TG. Pretreatment with colchicine 
      and cytochalasin D, drugs disrupting cytoskeleton, had no effect on a rise of 
      [Ca2+]i induced by TG. Genistein and erbastatin analog, inhibitors of tyrosine 
      kinase, inhibited an elevation of [Ca2+]i evoked by TG in a dose-dependent 
      manner. The present results suggest that tyrosine kinase regulates capacitative 
      Ca2+ entry into rat glioma C6 cells.
FAU - Takemura, H
AU  - Takemura H
AD  - Department of Pharmacology, School of Medicine, Sapporo Medical University, 
      Japan.
FAU - Imoto, K
AU  - Imoto K
FAU - Sakano, S
AU  - Sakano S
FAU - Kaneko, M
AU  - Kaneko M
FAU - Ohshika, H
AU  - Ohshika H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Res Commun Mol Pathol Pharmacol
JT  - Research communications in molecular pathology and pharmacology
JID - 9437512
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - H2D2X058MU (Cyclic GMP)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Cyclic GMP/metabolism
MH  - Cytoskeleton/drug effects/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - GTP-Binding Proteins/metabolism
MH  - Glioma/enzymology/*metabolism
MH  - Nitric Oxide/physiology
MH  - Pertussis Toxin
MH  - Phosphoprotein Phosphatases/antagonists & inhibitors/metabolism
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/*metabolism
MH  - Rats
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1998/02/19 00:00
MHDA- 1998/02/19 00:01
CRDT- 1998/02/19 00:00
PHST- 1998/02/19 00:00 [pubmed]
PHST- 1998/02/19 00:01 [medline]
PHST- 1998/02/19 00:00 [entrez]
PST - ppublish
SO  - Res Commun Mol Pathol Pharmacol. 1997 Nov;98(2):127-40.