PMID- 9469451
OWN - NLM
STAT- MEDLINE
DCOM- 19980226
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 160
IP  - 4
DP  - 1998 Feb 15
TI  - RANTES activation of phospholipase D in Jurkat T cells: requirement of 
      GTP-binding proteins ARF and RhoA.
PG  - 1894-900
AB  - The chemokine RANTES is a potent agonist of T cell activation. In an 
      investigation of signal-transduction events activated by this chemokine, we have 
      shown that RANTES stimulates dose-dependent phospholipase D (PLD) activity in 
      Jurkat cells. Equilibrium-binding analyses using 125I-labeled RANTES indicated 
      the presence of a receptor for RANTES on these cells, which has a Kd of 0.1 nM, 
      is expressed at approximately 600 sites per cell, and a binding specificity that 
      was not comparable with that of any of the known chemokine receptors, since 
      125I-labeled RANTES was displaced by macrophage-inflammatory protein-1 beta (but 
      not macrophage-inflammatory protein-1 alpha), monocyte-chemotactic protein-1 
      (MCP-1), MCP-3, MCP-4, and eotaxin. RANTES-induced PLD activation was augmented 
      by GTP gamma S, but not GDP beta S, and inhibited by the protein kinase C 
      inhibitor bisindolylmaleimide, as well as the fungal metabolite brefeldin A, and 
      C3 exoenzyme (Clostridium botulinum), implicating the activation of RhoA. RANTES 
      also induced GTP-GDP exchange of immunoprecipitated RhoA. RANTES-stimulated PLD 
      activity was dependent on an ADP-ribosylation factor(s), as assessed by 
      inhibition studies using a synthetic inhibitory peptide of the N-terminal 16 
      amino acids of ADP-ribosylation factor 1. These studies indicate the potential 
      existence of a novel receptor-mediated mechanism for activation of T cells by the 
      chemokine RANTES.
FAU - Bacon, K B
AU  - Bacon KB
AD  - Department of Immunobiology, DNAX Research Institute, Palo Alto, CA 94304, USA. 
      Kbacon@neurocrine.com
FAU - Schall, T J
AU  - Schall TJ
FAU - Dairaghi, D J
AU  - Dairaghi DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL5)
RN  - 0 (Cyclopentanes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Spiro Compounds)
RN  - 152697-41-1 (diepoxin sigma)
RN  - 20350-15-6 (Brefeldin A)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - ADP-Ribosylation Factors
MH  - Amino Acid Sequence
MH  - Brefeldin A
MH  - Chemokine CCL5/antagonists & inhibitors/*pharmacology
MH  - Cyclopentanes/pharmacology
MH  - Drug Synergism
MH  - Enzyme Activation/drug effects
MH  - Epoxy Compounds/pharmacology
MH  - GTP-Binding Proteins/*physiology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/physiology
MH  - Humans
MH  - Jurkat Cells
MH  - Molecular Sequence Data
MH  - Phospholipase D/antagonists & inhibitors/*metabolism
MH  - Protein Binding
MH  - Protein Kinase C/physiology
MH  - Spiro Compounds/pharmacology
MH  - T-Lymphocytes/drug effects/*enzymology
MH  - rhoA GTP-Binding Protein
EDAT- 1998/02/20 00:00
MHDA- 1998/02/20 00:01
CRDT- 1998/02/20 00:00
PHST- 1998/02/20 00:00 [pubmed]
PHST- 1998/02/20 00:01 [medline]
PHST- 1998/02/20 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Feb 15;160(4):1894-900.