PMID- 9471983
OWN - NLM
STAT- MEDLINE
DCOM- 19980327
LR  - 20220317
IS  - 1046-199X (Print)
IS  - 1046-199X (Linking)
VI  - 9
IP  - 1
DP  - 1998 Mar
TI  - A histological and immunohistochemical study on chronic irritant contact 
      dermatitis.
PG  - 23-8
AB  - BACKGROUND: Chronic irritant contact dermatitis (CICD) is characterized by 
      erythema, scaling, hyperkeratosis, chapping and fissures. It may be the result of 
      skin damage evoked by the cumulative effect of a variety of irritant stimuli. The 
      diagnosis of CICD is made on basis of the patient's history and clinical 
      features. No specific diagnostic tests are available. OBJECTIVE: The 
      histopathologic and cell biological features of CICD have not been extensively 
      studied. Here, we describe the histological and immunohistological changes in 
      CICD. METHODS: Punch biopsies were taken from 11 patients with CICD for 
      hematoxylin eosin and immunohistochemical stainings. Four skin biopsies of the 
      palms of the hands of healthy volunteers served as controls. RESULTS: The 
      histopathologic pattern was characterized by different grades of hyperkeratosis, 
      parakeratosis, spongiosis, exocytosis, acanthosis, and mononuclear perivascular 
      infiltrates. Mitotic activity, as measured by Ki-67-staining in the epidermis, 
      was increased fourfold in involved skin as compared with normal skin. Involucrin, 
      a structural protein of the cornified envelope, was expressed from the stratum 
      granulosum throughout the stratum spinosum in all patients with CICD and was 
      upregulated in comparison with normal skin. Epidermal fatty-acid binding protein 
      (E-FABP), a terminal differentiation marker, was proportionally unaltered in the 
      CICD as compared with the normal skin and was localized from the stratum 
      granulosum to the upper layers of the stratum spinosum. Cytokeratin 16, a 
      differentiation marker expressed in hyperproliferative epidermis, was markedly 
      increased from the stratum granulosum throughout the stratum spinosum in 5 out of 
      11 patients with CICD. Skin-derived antiproteinase (SKALP)/elafin, a proteinase 
      inhibitor expressed in inflamed epidermis, was only detected within the stratum 
      granulosum in 3 out of 11 patients. CONCLUSION: We conclude that CICD is 
      clinically characterized by features of a chronic dermatitis and, at the 
      histological level, by inflammatory changes, epidermal hyperproliferation and 
      altered differentiation.
FAU - Le, T K
AU  - Le TK
AD  - Department of Dermatology, University Hospital Nijmegen, The Netherlands.
FAU - Schalkwijk, J
AU  - Schalkwijk J
FAU - van de Kerkhof, P C
AU  - van de Kerkhof PC
FAU - van Haelst, U
AU  - van Haelst U
FAU - van der Valk, P G
AU  - van der Valk PG
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Am J Contact Dermat
JT  - American journal of contact dermatitis : official journal of the American Contact 
      Dermatitis Society
JID - 9100472
RN  - 0 (Carrier Proteins)
RN  - 0 (FABP5 protein, human)
RN  - 0 (FABP7 protein, human)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Myelin P2 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
RN  - 0 (Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 60108-77-2 (involucrin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Carrier Proteins/analysis
MH  - Cell Division
MH  - Chronic Disease
MH  - Dermatitis, Irritant/metabolism/*pathology
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Myelin P2 Protein/analysis
MH  - *Neoplasm Proteins
MH  - Protein Precursors/analysis
MH  - Proteinase Inhibitory Proteins, Secretory
MH  - Proteins/analysis
MH  - Skin/chemistry/*pathology
MH  - *Tumor Suppressor Proteins
EDAT- 1998/04/04 00:00
MHDA- 1998/04/04 00:01
CRDT- 1998/04/04 00:00
PHST- 1998/04/04 00:00 [pubmed]
PHST- 1998/04/04 00:01 [medline]
PHST- 1998/04/04 00:00 [entrez]
AID - S1046199X98000037 [pii]
PST - ppublish
SO  - Am J Contact Dermat. 1998 Mar;9(1):23-8.