PMID- 9472620
OWN - NLM
STAT- MEDLINE
DCOM- 19980226
LR  - 20081121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 79 ( Pt 2)
DP  - 1998 Feb
TI  - Herpes simplex virus type 1 glycoproteins gB, gC and gD are major targets for CD4 
      T-lymphocyte cytotoxicity in HLA-DR expressing human epidermal keratinocytes.
PG  - 353-61
AB  - T lymphocytes are the main mediators of the protective immune response in 
      recurrent herpes simplex. Early in the development of recurrent lesions, 
      macrophages and CD4 T lymphocytes predominate in the mononuclear infiltrate 
      surrounding infected epidermal cells. Human epidermal keratinocytes allow herpes 
      simplex virus type 1 (HSV-1) replication and human leukocyte antigen (HLA)-DR is 
      strongly expressed in vivo. In vitro, their pretreatment with IFN-gamma induced 
      HLA-DR expression and partially reversed major histocompatibility complex class I 
      down-regulation by the virus. Mononuclear cell cytotoxicity for these cells was 
      mediated predominantly by CD4 and also by CD8 T cells. Late HSV-1 proteins were 
      the major targets for CD4 CTL, while CD8 CTL predominantly targeted early HSV-1 
      proteins. Here it is shown that both mononuclear and CD4 CTL consistently 
      recognized the major HSV-1 glycoproteins, gB, gC, gD and gH, using 
      IFN-gamma-pretreated keratinocytes infected with vaccinia virus-HSV glycoprotein 
      recombinants (VvgB, VvgC, VvgD or VvgH). CD4 cytotoxicity was highest for 
      VvgD-infected keratinocytes, followed by VvgB or VvgC and then VvgH in seven 
      patients. CD4 CTL from two of 13 patients also recognized an epitope in the HSV 
      tegument protein VP16, demonstrated by comparing cytotoxicity for the partial 
      deletion mutants RP3 or RP4 and the parental RP1 HSV strain. In summary, the 
      major HSV glycoproteins gB, gC and gD were consistently the major targets for CD4 
      CTL in VvgB-, VvgC-, VvgD- and VvgH-infected, IFN-gamma-pretreated human 
      epidermal keratinocytes in vitro.
FAU - Mikloska, Z
AU  - Mikloska Z
AD  - Centre for Virus Research, Westmead Institutes for Health Research, Westmead 
      Hospital, Australia.
FAU - Cunningham, A L
AU  - Cunningham AL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein B, Simplexvirus)
RN  - 0 (glycoprotein D, Human herpesvirus 1)
RN  - 0 (glycoprotein gC, herpes simplex virus type 1)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Cytotoxicity, Immunologic
MH  - HLA-DR Antigens/*biosynthesis
MH  - Herpes Simplex/*immunology
MH  - Herpesvirus 1, Human/immunology/*physiology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Keratinocytes/drug effects/*immunology/virology
MH  - Killer Cells, Natural/immunology
MH  - Recombinant Proteins/biosynthesis
MH  - Skin/*immunology/virology
MH  - T-Lymphocyte Subsets/immunology/virology
MH  - Transfection
MH  - Viral Envelope Proteins/*biosynthesis
MH  - *Virus Replication
EDAT- 1998/02/24 00:00
MHDA- 1998/02/24 00:01
CRDT- 1998/02/24 00:00
PHST- 1998/02/24 00:00 [pubmed]
PHST- 1998/02/24 00:01 [medline]
PHST- 1998/02/24 00:00 [entrez]
AID - 10.1099/0022-1317-79-2-353 [doi]
PST - ppublish
SO  - J Gen Virol. 1998 Feb;79 ( Pt 2):353-61. doi: 10.1099/0022-1317-79-2-353.