PMID- 9472675
OWN - NLM
STAT- MEDLINE
DCOM- 19980303
LR  - 20240316
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 111
IP  - 1
DP  - 1998 Jan
TI  - IL-4, IL-10 and IL-13 down-regulate monocyte-chemoattracting protein-1 (MCP-1) 
      production in activated intestinal epithelial cells.
PG  - 152-7
AB  - Several studies have demonstrated that intestinal epithelial cells play a major 
      role in the initiation and perpetuation of intestinal inflammation by secreting 
      proinflammatory cytokines and chemokines. MCP-1 is suggested to be a chemokine 
      that plays a major part during intestinal inflammation in inflammatory bowel 
      disease (IBD). Immunoregulatory cytokines such as IL-4, IL-10 and IL-13 have been 
      described to exert anti-inflammatory properties on various cell types. The aim of 
      our study was to determine the effect of Th2 cytokines on the production of MCP-1 
      by activated intestinal epithelial cells. We examined Caco-2 cells as well as 
      intestinal epithelial cells which were isolated from surgical specimens. 
      Production of the chemokine MCP-1 was determined under stimulated and 
      non-stimulated conditions. IL-4, IL-10 and IL-13 were added to stimulated 
      epithelial cells under various culture conditions. Supernatants were analysed for 
      cytokine concentrations using ELISAs. Under stimulation with physiological agents 
      like IL-1beta or tumour necrosis factor-alpha (TNF-alpha), we observed markedly 
      increased concentrations of MCP-1 in supernatants of Caco-2 cells and intestinal 
      epithelial cells. IL-4, IL-10 and IL-13 all had the capacity to down-regulate the 
      production of MCP-1 in Caco-2 cells as well as in freshly isolated epithelial 
      cells. Caco-2 cells which were primed with Th2 cytokines 24 h before stimulation 
      were subsequently decreased in their ability to be stimulated by IL-1beta or 
      TNF-alpha for MCP-1 production. As MCP-1 has been shown to play a major role 
      during intestinal inflammation, the in vitro suppression of MCP-1 in enterocytes 
      suggests the in vivo use of regulatory cytokines in patients with active IBD.
FAU - Kucharzik, T
AU  - Kucharzik T
AD  - Department of Medicine B, University of Munster, Germany.
FAU - Lugering, N
AU  - Lugering N
FAU - Pauels, H G
AU  - Pauels HG
FAU - Domschke, W
AU  - Domschke W
FAU - Stoll, R
AU  - Stoll R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-13)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Caco-2 Cells
MH  - Chemokine CCL2/*biosynthesis
MH  - Down-Regulation
MH  - Humans
MH  - Interleukin-10/*pharmacology
MH  - Interleukin-13/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Intestinal Mucosa/immunology/*metabolism
PMC - PMC1904856
EDAT- 1998/02/24 00:00
MHDA- 1998/02/24 00:01
PMCR- 1999/01/01
CRDT- 1998/02/24 00:00
PHST- 1998/02/24 00:00 [pubmed]
PHST- 1998/02/24 00:01 [medline]
PHST- 1998/02/24 00:00 [entrez]
PHST- 1999/01/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1998.00481.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1998 Jan;111(1):152-7. doi: 10.1046/j.1365-2249.1998.00481.x.