PMID- 9472938
OWN - NLM
STAT- MEDLINE
DCOM- 19980310
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 58
IP  - 1
DP  - 1998 Jan
TI  - Messenger ribonucleic acid encoding monocyte chemoattractant protein-1 is 
      expressed by the ovine corpus luteum in response to prostaglandin F2alpha.
PG  - 169-74
AB  - To investigate expression of monocyte chemoattractant protein-1 (MCP-1) in the 
      ovine corpus luteum, a partial cDNA was produced by reverse 
      transcription-polymerase chain reaction. This cDNA was 89% identical to that 
      reported for bovine MCP-1 mRNA. In experiment 1, steady-state concentrations of 
      mRNA encoding MCP-1 were measured in pools of luteal tissue collected on Days 3, 
      6, 9, 12, and 15 of the estrous cycle (estrus = O; n = 4/day). There were no 
      differences in mRNA concentrations for MCP-1 among any of the days studied (p = 
      0.43). In experiment 2, midluteal-phase corpora lutea were collected from ewes at 
      0 (untreated), 2, 4, 8, and 16 h after administration of a luteolytic dose of 
      prostaglandin F2alpha (PGF2alpha; n = 4/time point). Concentrations of MCP-1 mRNA 
      were undetectable in untreated controls, were detectable at 2 h post-treatment, 
      had increased 4 and 8 h after administration of PGF2alpha when compared to those 
      at 2 h (p < 0.05), and were decreased 16 h after administration of PGF2alpha when 
      compared to those at 4 h (p < 0.05). In situ hybridization for MCP-1 mRNA 
      combined with immunocytochemical labeling of tissue inhibitor of 
      metalloproteinase-1 (TIMP-1) in large luteal cells was used to determine whether 
      the steroidogenic cells that have PGF2alpha receptors express MCP-1 mRNA in 
      response to PGF2alpha. Messenger RNA encoding MCP-1 and TIMP-1 were not 
      colocalized, indicating that MCP-1 was not expressed by large steroidogenic 
      luteal cells during luteolysis.
FAU - Haworth, J D
AU  - Haworth JD
AD  - Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort 
      Collins 80523, USA.
FAU - Rollyson, M K
AU  - Rollyson MK
FAU - Silva, P
AU  - Silva P
FAU - McIntush, E W
AU  - McIntush EW
FAU - Niswender, G D
AU  - Niswender GD
LA  - eng
GR  - HD11590/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (prostaglandin F2alpha receptor)
RN  - B7IN85G1HY (Dinoprost)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Chemokine CCL2/*genetics
MH  - Corpus Luteum/drug effects/*metabolism
MH  - Dinoprost/*pharmacology
MH  - Female
MH  - Gene Expression/*drug effects
MH  - RNA, Messenger/*metabolism
MH  - Receptors, Prostaglandin/analysis
MH  - *Sheep
EDAT- 1998/02/24 00:00
MHDA- 1998/02/24 00:01
CRDT- 1998/02/24 00:00
PHST- 1998/02/24 00:00 [pubmed]
PHST- 1998/02/24 00:01 [medline]
PHST- 1998/02/24 00:00 [entrez]
AID - 10.1095/biolreprod58.1.169 [doi]
PST - ppublish
SO  - Biol Reprod. 1998 Jan;58(1):169-74. doi: 10.1095/biolreprod58.1.169.