PMID- 9474018
OWN - NLM
STAT- MEDLINE
DCOM- 19980316
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 44
IP  - 2
DP  - 1998 Feb
TI  - Direct enzyme immunometric measurement of plasma big endothelin-I concentrations 
      and correlation with indicators of left ventricular function.
PG  - 239-43
AB  - Recent studies have suggested that the plasma concentrations of endothelin-1, a 
      potent vasoconstrictive peptide, are increased in patients with congestive heart 
      failure. This study aimed to evaluate a new direct ELISA for big endothelin-1 
      (the precursor of endothelin-1), in comparison with a big endothelin-1 ELISA 
      using plasma sample extraction, and to investigate whether plasma big 
      endothelin-1 concentrations correlate with indicators of left ventricular 
      function. The direct ELISA yielded significantly (P < 0.001) lower results than 
      the assay with extracted samples (0.9 +/- 0.5 pmol/L vs 2.7 +/- 1.9 pmol/L; n = 
      90); however, the results of the two assays were closely correlated (r = 0.86, P 
      < 0.001). Plasma big endothelin-1 concentrations exhibited a significant (P < 
      0.001) negative correlation (r = -0.46, r = -0.40) with the left ventricular 
      ejection fraction and a significant positive correlation (r = 0.40, P < 0.001; r 
      = 0.36, P < 0.01) with the left ventricular end-diastolic pressure and the left 
      ventricular end-diastolic (r = 0.42, r = 0.38, P < 0.001) and end-systolic (r = 
      0.52, r = 0.47, P < 0.001) volume indices. Plasma big endothelin-1 concentrations 
      were notably greater in patients with New York Heart Association (NYHA) class 
      II-IV symptoms than in patients without cardiac disease or in patients 
      categorized to NYHA class I. These data suggest that plasma big endothelin-1 
      concentrations, measured by a direct ELISA, correlate with hemodynamic indicators 
      and symptoms of left ventricular dysfunction.
FAU - Haug, C
AU  - Haug C
AD  - Institute of Clinical Chemistry, University Hospital, Ulm, Germany. 
      cornelia.haug@medizin.uni-ulm.de
FAU - Koenig, W
AU  - Koenig W
FAU - Hoeher, M
AU  - Hoeher M
FAU - Kochs, M
AU  - Kochs M
FAU - Hombach, V
AU  - Hombach V
FAU - Gruenert, A
AU  - Gruenert A
FAU - Osterhues, H
AU  - Osterhues H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Endothelin-1)
RN  - 0 (Endothelins)
RN  - 0 (Protein Precursors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Endothelin-1
MH  - Endothelins/*blood
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Heart Diseases/blood
MH  - Hemodynamics/physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Protein Precursors/*blood
MH  - Statistics as Topic
MH  - Ventricular Function, Left/*physiology
EDAT- 1998/02/25 00:00
MHDA- 1998/02/25 00:01
CRDT- 1998/02/25 00:00
PHST- 1998/02/25 00:00 [pubmed]
PHST- 1998/02/25 00:01 [medline]
PHST- 1998/02/25 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1998 Feb;44(2):239-43.