PMID- 9475399
OWN - NLM
STAT- MEDLINE
DCOM- 19980330
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 58
IP  - 2
DP  - 1998 Feb
TI  - In vitro regulation of an inducible-type NO synthase in the rat seminiferous 
      tubule cells.
PG  - 431-8
AB  - Rat Sertoli cells express an inducible nitric oxide synthase isoform (iNOS) in 
      response to the combined addition of the cytokines--interferon gamma (IFNgamma), 
      tumor necrosis factor alpha (TNF alpha), interleukin-1alpha (IL-1alpha)--and 
      lipopolysaccharides (LPS). We demonstrated that the addition of cytokines and 
      lipopolysaccharides (C+L) to cultured peritubular cells resulted in high nitrite 
      and iNOS mRNA levels, indicating the induction of an iNOS isoform. This enzyme 
      was not induced in cultured pachytene spermatocytes or spermatids. Nitrite 
      production in Sertoli cells and peritubular cells required both IFNgamma and TNF 
      alpha and was potentiated by LPS, whereas IL-1alpha was ineffective. The 
      induction of nitrite production and iNOS mRNA by IFNgamma+TNF alpha+LPS could be 
      further enhanced by basic fibroblast growth factor in Sertoli cells but not in 
      peritubular cells. In contrast, transforming growth factor beta markedly reduced 
      this induction in peritubular cells but had no effect on Sertoli cells. FSH 
      positively modulated the C+L-induced iNOS in Sertoli cells. Dibutyryl cAMP had a 
      synergistic effect with C+L on NOS activity in both Sertoli cells and peritubular 
      cells. In contrast, testosterone did not influence basal or induced NOS activity 
      in these two cell types. These data show that NOS activity in the somatic cells 
      of the seminiferous tubules is induced and regulated by multiple factors that act 
      in combination, and suggest that nitric oxide may participate in the endocrine 
      and paracrine control of testicular function.
FAU - Bauche, F
AU  - Bauche F
AD  - Groupe d'Etude de la Reproduction chez le Male, INSERM U-435, Universite de 
      Rennes I, Bretagne, France.
FAU - Stephan, J P
AU  - Stephan JP
FAU - Touzalin, A M
AU  - Touzalin AM
FAU - Jegou, B
AU  - Jegou B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Cytokines)
RN  - 0 (Hormones)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
SB  - IM
MH  - Animals
MH  - Cytokines/pharmacology
MH  - Enzyme Induction/drug effects
MH  - Germ Cells/drug effects/enzymology
MH  - Hormones/pharmacology
MH  - Indicators and Reagents
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Nitric Oxide Synthase/*biosynthesis
MH  - Nitric Oxide Synthase Type II
MH  - RNA, Messenger/biosynthesis/isolation & purification
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/cytology/drug effects/*enzymology
MH  - Sertoli Cells/drug effects/enzymology
MH  - Testis/drug effects/enzymology
EDAT- 1998/02/25 00:00
MHDA- 1998/02/25 00:01
CRDT- 1998/02/25 00:00
PHST- 1998/02/25 00:00 [pubmed]
PHST- 1998/02/25 00:01 [medline]
PHST- 1998/02/25 00:00 [entrez]
AID - 10.1095/biolreprod58.2.431 [doi]
PST - ppublish
SO  - Biol Reprod. 1998 Feb;58(2):431-8. doi: 10.1095/biolreprod58.2.431.